Long-term ingestion of phenytoin elicits gingival overgrowth. We investigated sulfated glycosaminoglycan (GAG) metabolism by human gingival fibroblasts from normal gingivae and from phenytoin-enlarged gingivae. Incorporation, utilization, and subsequent loss of 35SO4= was measured in pulse-chase experiments. Fibroblasts from overgrown gingivae demonstrated increased accumulation of sulfated glycosaminoglycans. The increase was due to elevated synthesis and not to decreased degradation.
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