The distribution of lipids in the fractions of parotid and submandibular saliva following Bio-Gel A-50 column chromatography was measured. Over 50% of the total lipids of submandibular saliva was found in the fraction which contained mainly the high-molecular-weight glycoprotein. This fraction also contained most of the glycolipids, free fatty acids, phospholipids, and cholesterol. In the parotid saliva, the fraction containing the basic glycoprotein (the major glycoprotein fraction of parotid saliva) contained 35% of the total saliva lipids and was enriched in phospholipids ana cholesterol esters.
Get full access to this article
View all access options for this article.
References
1.
Mandel, I.D. : Human Submaxillary, Sublingual, and Parotid Glycoproteins and Enamel Pellicle . In: The Glycoconjugate, Vol. I, Horowitz, M.I. and Pigman, W., Eds., New York: Academic Press, 1977, pp. 153-179.
2.
Dirksen, T.R. : Lipid Constituents of Whole and Parotid Saliva , J Dent Res42: 920-924, 1963.
3.
Levine, M.J. ; Ellison, S.A.; and Bahl, O.P.: The Isolation from Human Parotid Saliva and the Partial Characterization of the Protein Core of a Major Parotid Glycoprotein , Arch Oral Biol18: 827-837, 1973.
4.
Li, H.C. and Levine, M.J.: Characterization of a Glycopeptide from the Proline-Rich Glycoprotein of Human Parotid Saliva, Arch Oral Biol25:353-355, 1980.
5.
Mandel, I.D. and Eisenstein, A.: Lipids in Human Salivary Secretions and Salivary Calculi, Arch Oral Biol14: 231-233, 1969.
6.
Rabinowitz, J.L. and Shannon, I.L.: Lipid Changes in Human Male Parotid Saliva by Stimulation, Arch Oral Biol20: 403-406, 1975.
7.
Slomiany, B.L. ; Slomiany, A.; and Mandel, I.D.: Lipid Composition of Human Submandibular Gland Secretion from Light and Heavy Calculus Formers , Arch Oral Biol25 :749-751,1980.
8.
Slomiany, A. ; Slomiany, B.L.; and Mandel, I.D.: Lipid Composition of Human Parotid Saliva from Light and Heavy Dental Calculus-Formers, Arch Oral Biol26:151-152, 1981.
9.
Anneroth, G. ; Eneroth, C.M.; and Isacsson , G.: The Relation of Lipids to the Mineral Components in Salivary Calculi, J Oral Path6:373-381, 1977.
10.
Boskey, A.L. ; Boyan-Salyers, L.; Burstein , L.S.; and Mandel, I.D.: Lipids Associated with Mineralization of Human Submandibular Gland Sialoliths, Arch Oral Biol26: 779-785, 1981.
11.
Johnson, A.R. : Observations on Calcium-induced Stability of Proteins in a Lipid-extraction System, J Dent Res55 :470-475, 1976.
12.
Voegel, J.C. and Belcourt, A.: Influence of Calcium and Other Cations on the Precipitation of Human Salivary Glycoprotein and the Nature of the Mineral Phase of the Precipitates, Arch Oral Biol25:137-139, 1980.
13.
Mantle, M. and Allen, A.: A Colorimetric Assay for Glycoproteins Based on the Periodic Acid/Schiff Stain , Biochem Soc Trans6:607-609, 1978.
14.
Slomiany, A. ; Yano, S.; Slomiany , B.L.; and Glass, G.B.J.: Lipid Composition of the Gastric Mucous Barrier in the Rat, J Biol Chem253:3785-3791, 1978.
15.
Lowry, R.R. and Tinsley, I.J.: A Simple Sensitive Method for Lipid Phosphorus , Lipids9:491-492, 1974.
16.
Prakobphol, A.; Levine, M.J.; Tabak , L.A.; and Red-Dy , M.S.: Purification of a Low Molecular Weight Mucin-Glycoprotein from Human Submandibular-Sublingual Saliva, Carbohydr Res108:111-122, 1982.
17.
Mandel, I.D. : In Defense of the Oral Cavity. In: Saliva and Dental Caries , Kleinberg, I., Ellison, S.A., and Mandel, I.D., Eds., Microbiology Abstracts, Special Supplement, 1979, pp. 473-492.
18.
Orstavik, D. and Kraus, F.W.: The Acquired Pellicle: Enzyme and Antibody Activities, Scand J Dent Res32 :202-205, 1974.
19.
Martin, G.P. ; Marriot, C.; and Kellaway, I.W.: Direct Effect of Bile Salts and Phospholipids on the Physical Properties of Mucus, Gut19:103-107, 1978.
20.
Siegel, LA and Izutsu, K.T.: Permeability of Oral Mucosa to Organic Compounds, J Dent Res59:1604-1605, 1980.
21.
Schachtele, C.F.; Harlander, S.K.; Bracke , J.W.; Ostrum, L.C.; Maltais, J.B.; and Billings , R.J.: Streptococcus mutans Dextransucrase: Stimulation by Phospholipids from Human Sera and Oral Fluids , Infect Immun22:714-720, 1978.
22.
Beachey, E.H. : Bacterial Adherence: Adhesion-Receptor Interactions Mediating the Attachment of Bacteria to Mucosal Surfaces, J Infect Dis143:325-345, 1981.
23.
Slomiany, B.L. ; Banas-Gruszka, Z.; and Slomiany, A.: Association of Glyceroglucolipids with Glycoproteins of Gastric Mucus, IRCS Med Sci9:757, 1981.
