The protein matrix of fetal human dental enamel was isolated and fractionated by chromatographic and electrophoretic procedures. Nine principal protein fractions were isolated and characterized. Possible inter-relationships of these proteins and their comparison with data from other species were examined. Significant differences between human proteins and those described for cow or pig enamel were identified.
Get full access to this article
View all access options for this article.
References
1.
Deakins, M.: Changes in Ash, Water and Organic Content of Pig Enamel During Calcification , J Dent Res21:429-436, 1942.
2.
Eastoe, J.E. : Enamel Protein Chemistry - Past, Present and Future, J Dent Res58B:753-763, 1979.
3.
Termine, J.D. ; Belcourt, A.B.; Christner , P.J.; Conn, K.M.; and Nylen, M.U.: Properties of Dissociatively Extracted Fetal Tooth Matrix Proteins. I. Principal Molecular Species in Developing Bovine Enamel, J Biol Chem255 :9760-9768, 1980.
4.
Belcourt, A.B. ; Fincham, A.G.; and Termine, J.D.: Ethylenediaminetetraacetic Acid Insoluble Protein of Adult Human Enamel, Caries Res16:72-76, 1982.
5.
Robinson, C. ; Briggs, H.D.; Atkinson , P.J.; and Weatherell , J.A.: Matrix and Mineral Changes in Developing Enamel, J Dent Res58B:871-880, 1979.
6.
Fincham, A.G. ; Belcourt, A.B.; and Termine, J.D.: Changing Patterns of Enamel Matrix Proteins in the Developing Bovine Tooth, Caries Res16:64-71, 1982.
7.
Katz, E.P.; Mechanic, G.L.; and Glimcher, M.J.: The Ultracentrifugal and Free Zone Electrophoretic Characterization of the Neutral Soluble Proteins of Embryonic Bovine Enamel, Biochim Biophys Acta107:471-484, 1965.
8.
Burgess, R.C. and Maclaren, C.M.: Proteins in Developing Bovine Enamel. In: Tooth Enamel I, Stack, M.V. and Fearnhead, R.W., Eds., Bristol, England: Wright Bros., 1965, pp. 74-82.
9.
Fincham, A.G. : Electrophoretic and Sephadex Gel Filtration Studies of Bovine Fetal Enamel Matrix at Acid pH, Calcif Tissue Res2:353-360, 1968.
10.
Eggert, F.M. ; Allen, G.A.; and Burgess, R.C.: Amelogenins - Purification and Partial Characterization of Proteins from Developing Bovine Dental Enamel, Biochem J131 :471-484, 1973.
11.
Fukae, J. and Shimizu, M.: Studies on the Proteins of Developing Bovine Enamel, Arch Oral Biol19:381-386, 1974.
12.
Seyer, J. and Glimcher, M.J.: Evidence for the Presence of Numerous Protein Components in Immature Bovine Dental Enamel, Calcif Tissue Res24:253-257, 1977.
13.
Fincham, A.G. : The Amelogenin Problem: A Comparison of Purified Enamel Matrix Proteins, Calcif Tissue Intl27:65-73, 1979.
14.
Fukae, M.; Ijiri, H.; Tanabe, T.; and Shimizu, M.: Partial Amino Acid Sequences of Two Proteins in Developing Porcine Enamel , J Dent Res58B:1000-1001, 1979 .
15.
Fukae, M.; Tanabe, T.; Ijiri, H.; and Shimizu, M.: Studies of Porcine Enamel Proteins: A Possible Original Enamel Protein , Tsurumi Univ Dent J6:87-94, 1980.
16.
Hiller, C.R. ; Robinson, C.; and Weatherell , J.A.: Variations in the Composition of Developing Rat Incisor Enamel, Calcif Tissue Res18:1-12, 1975.
17.
Elwood, W.K. and Apostopoulos , A.X.: Analysis of Developing Enamel of the Rat. I. Fractionation: Protein and Calcium Content, Calcif Tissue Res17:317-326, 1975.
18.
Levine, P.T. ; Seyer, J.; Huddleston , J.; and Glimcher, M.J.: The Comparative Biochemistry of the Organic Matrix Proteins of Developing Enamel, Arch Oral Biol12 :407-410, 1967.
19.
Katz, E.P.; Seyer, J.; Levine, P.T.; and Glimcher, M.J.: The Comparative Biochemistry of the Organic Matrix of Developing Enamel. II. Ultracentrifugal and Electrophoretic Characterization of Proteins Soluble at Neutral pH, Arch Oral Biol14:533-539, 1969.
20.
Fincham, A.G. ; Belcourt, A.B.; Lyaruu , D.M.; and Termine, J.D.: Comparative Protein Biochemistry of Developing Dental Enamel Matrix from Five Mammalian Species, Calcif Tissue Intl34:182-189, 1982.
21.
Fincham, A.G. ; Burkland, G.A.; and Shapiro, I.M.: Lipophilia of Enamel Matrix. A Chemical Investigation of the
22.
Neutral Lipids and Lipophilic Proteins of Enamel, Calcif Tissue Res9:247-259, 1971.
23.
Reisner, A.H. ; Nemes, P.; and Bucholtz, C.: The Use of Coomassie Brilliant Blue G250 Perchloric Acid Solution for Staining in Electrophoresis and Isoelectric Focusing on Polyacrylamide Gels, Analyt Biochem64:509-516, 1975.
24.
Keutman, H.T. and Potts, J.T.: Improved Recovery of Methionine after Acid Hydrolysis Using Mercaptoethanol, Analyt Biochem29:175-185, 1969.
25.
Fincham, A.G. ; Belcourt, A.B.; Termine , J.D.; Butler, W.T.; and Cothran, W.C.: Dental Enamel Matrix: Sequences of Two Amelogenin Polypeptides, Bioscience Reports1:771-778, 1981.
26.
Belcourt, A.B. ; Fincham, A.G.; and Termine, J.D.: Bovine High Molecular Weight Amelogenin Proteins, Calcif Tissue Intl, In press, 1982 .
27.
Ungewickell, E. and Branton, D.: Assembly Units of Clathrin Coats , Nature289:420-422, 1981.
28.
Bretscher, A. and Weber, K.: Tropomyosin from Bovine Brain Contains Two Polypeptide Chains of Slightly Different Molecular Weights, FEBS Letts85:145-148, 1978.
29.
Lyaruu, D.M. ; Belcourt, A.B.; Fincham , A.G.; and Termine, J.D.: Neonatal Hamster Molar Tooth Development : Extraction and Characterization of Amelogenins , Enamelins and Soluble Dentin Proteins, Calcif Tissue Intl34: 86-96, 1982.
30.
Deutsch, D. and Gedalia, I.: Chemically Distinct Stages in Developing Human Fetal Enamel, Arch Oral Biol25 :635-639, 1980.
31.
Slavkin, H.C. ; Mino, W.; and Bringas, P., Jr.: The Biosynthesis and Secretion of Precursor Enamel Protein by Ameloblasts as Visualized by Autoradiography after Tryptophan Administration, Anat Rec185:289-312, 1976.
