This study demonstrated that human submandibular-sublingual saliva (HSMSL) provided a better substrate than did whole saliva or parotid saliva for the binding of Streptococcus sanguis in a glass adherence assay. Additional evidence indicated that the lower molecular weight salivary mucin in HSMSL was involved in these interactions. Mucin's sialic acid residues were found to play a major role in mediating the binding of certain strains of Streptococcus sanguis.
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References
1.
Gibbons, R.J. and Van Houte , J.: Bacterial Adherence in Oral Microbial Ecology, Ann Rev Microbiol29:19-44, 1975.
2.
Mandel, I.D.: Salivary Factors in Caries Prediction. In: Methods of Caries Prediction, Bibby, B.E., and Stein, R.J., Eds., Special Supplement of Microbial Abstracts, New York and London: Information Retrieval, Inc. , 1978, pp. 147-158.
3.
Levine, M.J. ; Herzberg, M.C.; Levine , M.S.; Ellison, S.A.; Stinson, M.W.; Li , H.C.; and Van Dyke , T.: Specificity of Salivary-Bacterial Interactions: Role of Terminal Sialic Acid Residues in the Interaction of Salivary Glycoproteins with Streptococcus sanguis and Streptococcus mutans , Infect Immun19:107-115, 1978.
4.
Tabak, L.A.; Levine, M.J.; Mandel, LD; and Ellison, S.A.: Role of Salivary Mucins in the Protection of the Oral Cavity, J Oral Path11:1-17, 1982.
5.
Van Houte, J.: Bacterial Adhesion in the Mouth. In: Dental Plaque and Surface Interactions in the Oral Cavity, Leach, S.A., Ed., London: Information Retrieval, Limited, 1980, pp. 69-100.
6.
Gibbons, R.J. and Van Houte , J.: Bacterial Adherence and the Formation of Dental Plaques. In: Bacterial Adherence , Beachey, E.H., Ed., New York and London: Chapman and Hall, 1980, pp. 61-104.
7.
McBride, B.C. and Gisslow, M.T.: Role of Sialic Acid in Saliva-Induced Aggregation of Streptococcus sanguis, Infect Immun18:35-40, 1977.
8.
Prakobphol, A.; Levine, M.J.; Tabak , L.A.; and Reddy, M.S.: Purification of a Low Molecular Weight Mucin-Glycoprotein from Human Submandibular-Sublingual Saliva, Carbohydr Res108:111-122, 1982.
9.
Clark, W.B. and Gibbons, R.J.: Influence of Salivary Components and Extracellular Polysaccharide Synthesis from Sucrose on the Attachment of Streptococcus mutans 6715 to Hydroxyapatite Surfaces, Infect Immun18:514-523, 1977.
10.
Appelbaum, B.; Golub, E.; Holt , S.C.; and Rosan, B.: In vitro Studies of Dental Plaque Formation: Adsorption of Oral Streptococci to Hydroxyapatite , Infect Immun25 :717-728, 1979.
11.
Rstavik, D. ; Kraus, F.W.; and Henshaw, L.C.: In vitro Attachment of Streptococci to the Tooth Surface, Infect Immun9:794-800, 1974.
12.
Van Houte, J.: Oral Bacterial Colonization: Mechanisms and Implications. In: Proceedings: Microbial Aspects of Dental Caries, Stiles, H.M., Loesche, W.J., and O'Brien, T.C., Eds., Washington, D.C.: Information Retrieval, Inc., 1976, pp. 3-32.
13.
Hamada, S. and Slade, H.D.: Biology, Immunology, and Cariogenicity of Streptococcus mutans, Microbiol Rev44: 331-384, 1980.
14.
Stinson, M.W. ; Jinks, D.C.; and Merrick, J.M.: Adherence of Streptococcus mutans and Streptococcus sanguis to Salivary Components Bound to Glass, Infect Immun32 :583-591, 1981.
15.
Rosan, B.: Antigens of Streptococcus sanguis, Infect Immun7:205-211, 1973.
16.
Carlsson, J. : Presence of Various Types of Non-Haemolytic Streptococci in Dental Plaque and in Other Sites of the Oral Cavity in Man , Odontol Revy18:55-74, 1967.
17.
Curby, W.A. : Device for Collection of Human Parotid Saliva , J Lab Clin Med41:493-496, 1953.
18.
Block, P. and Brotman, S.: A Method of Submaxillary Saliva Collection Without Cannulization, NY State Dent J28: 116-118, 1962.
19.
Shomers, J.P.; Tabak, L.A.; Levine, M.J.; Mandel, I.D.; and Ellison, S.A.: The Isolation of a Family of Cysteine-containing Phosphoproteins from Human Submandibular-Sublingual Saliva, JDent Res, In press , 1982.
20.
Levine, M.J. ; Herzberg, M.C.; Ellison, S.A.; Shomers, J.P.; and Sadowski , G.A.: Biochemical and Immunological Comparison of Monkey (Macaca arctoides) and Human Salivary Secretions , Comp Biochem Physiol60B:423-431, 1978.
21.
Murray, P.A. ; Levine, M.J.; Tabak, L.A.; and Reddy, M.S.: Specificity of Salivary-Bacterial Interactions: II. Evidence for a Lectin on Streptococcus sanguis With Specificity for a NeuAcα2,3Galβ1,3GalNAc Sequence, Biochem Biophys Res Commun106:390-396, 1982.
22.
Prakobphol, A.; Levine, M.J.; and Tabak, L.A.: Heterotypic Mucin Complexes in Saliva, Annual Meeting: The Society for Complex Carbohydrates, Abstract No. 81, 1981 .
23.
Shomers, J.P. ; Tabak, L.A.; Levine , M.J.; Mandel, I.D.; and Hay , D.I.: Properties of Cysteine-containing Phosphoproteins from Human Submandibular-Sublingual Saliva, J Dent Res61:397-399, 1982.
24.
Iacono, V.J. : MACKAY, B.J.; DIRIENZO, S.; and POL-LACK, J.J .: Selective Antibacterial Properties of Lysozyme for Oral Microorganisms, Infect Immun29:623-632, 1980.
