The micro method of disc electrophoresis is introduced for determining DNase activity in human saliva. Acid DNase activity in an elderly age group was found to be low, compared with that in a young age group. However, electrophoretic properties of materials obtained from young and elderly age groups did not vary significantly.
Get full access to this article
View all access options for this article.
References
1.
Meyer, J. and Necheles, H.: Studies in Old Age. The Clinical Significance of Salivary, Gastric and Pancreatic Secretion in the Aged, JAMA115:2050-2053, 1940.
2.
Meyer, J.; Golden, J.S.; Steiner, N.; and Necheles, H.: The Ptyalin Content of Human Saliva in Old Age, Am J Physiol119:600-602, 1937.
3.
Zollner, E.J. ; Klepsch, D.M.; Zahn , R.K.; and Knep-Per , R.: Deoxyribonucleases in Human Parotid Saliva, Enzyme19:60-64, 1975.
4.
Zollner, E.J. ; Klepsch, D.M.; and Zahn, R.K.: Different Deoxyribonucleases in Human Submandibular Saliva, J Dent Res53:1499, 1974.
5.
Allison, A.C.: The Biological Basis of Medicine, Vol. 1, Bittar, E.E., Ed., London and New York : Academic Press, 1969, pp. 207-242.
6.
Sreebny, L.M. ; Wanamaker, B.B.; and Robinovitch , M.: The DNase of the Parotid Gland, J Dent Res44:463-466, 1965.
7.
Mizuno, D.: Aging and Lysosome, Taisha9:295-299, 1972.
8.
Sakata, T.: Electrophoretic Studies of DNase in Proliferative and Tumorous Tissues , J Kurume Med Assoc44:858-868, 1981.
9.
Funakoshi, A. ; Wakasugi, H.; and Ibayashi, H.: Clinical Investigation of Serum Deoxyribonuclease. II. Clinical Studies of Serum Deoxyribonuclease Activity in Pancreatic Disease , Gastroenterol Jpn14:436-440, 1979.
10.
Ueda, T.: Studies on Deoxyribonucleases of Cow Snout Epidermis by the Method of Micro-Disc Electrophoresis, J Kurume Med Assoc42:1252-1267, 1979.
11.
Gornall, A.G. ; Bardawill, C.J.; and David, M.M.: Determination of the Biuret Reaction, J Biol Chem177 :751-766, 1949.
12.
Junqueira, L.C.U.; Toledo, A.M.S.; and Doine, A.I.: Digestive Enzymes in the Parotid and Submandibular Glands of Mammals, An Acad Braso Ciênc45 :629-643, 1973.
13.
Mizutani, T. and Mizutani, A.: Isolation of a Deoxyribonuclease from Bovine Parotid Gland, Chem Pharm Bull22:2723-2725, 1974.
14.
Lundblad, R.L. ; Bartlett, S.R.; Hornsby , T.E.; Hundson, J.D.; and Rose, C.H.: Immunologic Nonidentity of Parotid and Pancreatic Nucleases, J Dent Res52:396, 1973.
15.
Bogart, B.I. : The Effect of Aging on the Histochemistry of the Rat Submandibular Gland, J Gerontol22:372-375, 1967.
16.
Reiss, J. and Gershon, D.: Rat-Liver Superoxide Dismutase, Eur J Biochem63:617-623, 1976.
17.
Sharma, H.K. ; Prasanna, H.R.; and Rothsteins , M.: Altered Phosphoglycerate Kinase in Aging Rats, J Biol Chem255:5043-5050, 1980.
