The antimicrobial oxidizing agent hypothiocyanite ion (OSCN-) was measured in resting (drooling) and stimulated (expectorated) whole saliva. Stimulation of the saliva flow rate resulted in a rapid decrease in OSCN- concentration, whereas the thiocyanate ion (SCN—) concentration and peroxidase activity were increased. The decrease in OSCN — levels was greater than could be accounted for by dilution of the whole saliva volume. Assuming that the antimicrobial activity of the salivary peroxidase system is proportional to OSCN- concentration, this system may be more effective in resting saliva than in stimulated saliva.
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References
1.
Hamon, C.B. and Klebanoff, S.J.: A Peroxidase-mediated Streptococcus mitis Dependent Antimicrobial System in Saliva, JExpMed137:438-450, 1973.
2.
Hoogendoorn, H.: The Effect of Lactoperoxidase-Thiocyanate-Hydrogen Peroxide on the Metabolism of Cariogenic Microorganisms in vitro and in the Oral Cavity, Mouton, Den Haag, The Netherlands, 1974.
3.
Tenovuo, J.; Valtakoski, J.; and Knuuttila, M.L.E. : Antibacterial Activity of Lactoperoxidase Absorbed by Human Salivary Sediment and Hydroxyapatite, Caries Res11 :257-262,1977.
4.
Tenovuo, J. and Anttonen, T.: Peroxidase-catalyzed Hypothiocyanite Production in Human Salivary Sediment in Relation to Oral Health, Caries Res14:269-275, 1980.
5.
Smith, J.W.; Chwang, A.K.; and Thomas, E.L.: Lactoperoxidase Antimicrobial Action in Human Saliva, IADR Progr & Abst60: No. 509, 1981.
6.
Tenovuo, J.; Mansson-Rahemtulla, B.; Pruitt, K.M.; and Arnold, R.: Inhibition of Dental Plaque Acid Production by the Salivary Lactoperoxidase Antimicrobial System, Infect Immun34:208-214, 1981.
7.
Germaine, G.R. and Tellefson, L.M.: Effect of Human Saliva on Glucose Uptake by Streptococcus mutans and Other Oral Microorganisms, Infect Immun31 :598-607, 1981.
8.
Rotgans, J. and Hoogendoorn, H.: The Effect of Brushing with a Toothpaste Containing Amyloglucosidase and Glucose Oxidase on Dental Caries in Rats, Caries Res13 :150-153,1979.
9.
Koch, G. and Strand, G.: Effect of an Enzyme Dentifrice on Caries. A Two-year Clinical Pilot Study , Swed Dent J3: 9-13,1979.
10.
Muhlemann, H.R.; Schmid, R.; and Firestone, A.R.: Effect on Rat Caries of Endogenous and Exogenous Hydrogen Peroxide, Caries Res15:46-53, 1981.
11.
Thomas, E.L. ; Bates, K.P.; and Jefferson , M.M.: Peroxidase Antimicrobial System of Human Saliva: Requirements for Accumulation of Hypothiocyanite, J Dent Res60: 780-796,1981.
12.
Pruitt, K.M. ; Tenovuo, J.; Fleming, W.; and Adamson, M.: Limiting Factors for the Generation of Hypothiocyanite Ion, an Antimicrobial Agent, in Human Saliva, Caries Res, In press, 1981.
13.
Aune, T.M. and Thomas, E.L.: Accumulation of Hypothiocyanite Ion During Peroxidase-catalyzed Oxidation of Thiocyanate Ion, Eur J Biochem80:209-214, 1977.
14.
Hoogendoorn, H.; Piessens, J.P.; Scholtes, W.; and Stoddard, L.A.: Hypothiocyanite Ion; the Inhibitor Formed by the System Lactoperoxidase-Thiocyanate-Hydrogen Peroxide, Caries Res11:77-84, 1977.
15.
Thomas, E.L. : Lactoperoxidase-catalyzed Oxidation of Thiocyanate: The Equilibria Between Oxidized Forms of Thiocyanate, Biochemistry20:3273-3280, 1981.
16.
Hogg, D.M. and Jago, G.R.: The Antimicrobial Action of Lactoperoxidase. The Nature of the Bacterial Inhibitor , BiochemJ117:779-790, 1970.
17.
Kersten, H.W. ; Moorer, W.R.; and Wever, R.: Thiocyanate as a Cofactor in Myeloperoxidase Activity Against Streptococcus mutans, J Dent Res60:831-837, 1981.
18.
Thomas, E.L. ; Bates, K.P.; and Jefferson , M.M.: Hypothiocyanite Ion: Detection of the Antimicrobial Agent in Human Saliva, J Dent Res59:1466-1472, 1980.
Pruitt, K.M. ; Adamson, M.; and Arnold, R.: Lactoperoxidase Binding to Streptococci, Infect Immun25 :304-309,1979.
21.
Shindler, J.S. ; Childs, R.E.; and Bardsley , W.G.: Peroxidase of Human Cervical Mucus. Isolation and Characterization,EurJBiochem65:325-331, 1976.
22.
Azen, E.A.: Salivary Peroxidase Activity and Thiocyanate Concentration in Human Subjects with Genetic Variants of Salivary Peroxidase, Arch Oral Biol23:801-805, 1978.
23.
Tenovuo, J. and Valtakoski, J.: The Correlation Between Salivary Peroxidase Activity, Salivary Flow Rate, and the Oxidation-reduction Potentials of Human Saliva and Dental Plaque Suspensions, Acta Odont Scand34:169-176, 1976.
24.
Globerman, D.Y. and Kleinberg, I.: Intra-oral pO2 and Its Relation to Bacterial Accumulation on the Oral Tissues. In: Proceedings, Saliva and Dental Caries, Sp. Supp. Microbiology Abstracts, Kleinberg, I., Ellison, S.A. , and Mandel, I.D., Eds., 1979, pp. 275-292.
25.
Bjorck, L. and Claesson, 0.: Correlation Between Concentration of Hypothiocyanite and Antibacterial Effect of the Lactoperoxidase System Against Escherichia coli, J Dairy Sci63:919-922, 1980.
