Members of a family of acidic proteins taken from human submandibular-sublingual saliva were designated cysteine-containing phosphoproteins, since they could be distinguished from other salivary phosphoproteins by the presence of half-cystine. These molecules consisted of a single peptide chain of ~ 14,000 daltons. Their isoelectric points ranged from 4.3 to 5.9. Two groups (C2 and C3) were 0-phosphorylated. Their charge heterogeneity was apparently due to variations in content of phosphate and acidic and basic amino acids.
Masson, P.L. ; Carbonara, A.O.; and Heremans , J.F.: Studies on the Proteins of Human Saliva, Biochim Biophys Acta107:485-500, 1965,
3.
Gugler, E.; Pallavicini, J.C.; Swerdlow, H.; Zipkin, I.; and Di Sant'Agnese. P.A.: Immunological Studies of Submaxillary Saliva from Patients with Cystic Fibrosis and from Normal Children, J Pediatr73:548-559, 1968,
4.
Shomers, J.P. ; Tabak, L.A.; Levine , M.J.; Mandel, I.D.; and Ellison, S.A.: The Isolation of a Family of Cysteine-containing Phosphoproteins from Human Submandibular-Sublingual Saliva, J Dent Res, In press.
5.
Levine, M.J. ; Herzberg, M.C.; Levine , M.S.; Ellison, S.A.; Stinson, M.W.; Li , H.C.; and Van Dyke , T.: Specificity of Salivary-Bacterial Interactions: Role of Terminal Sialic Acid Residues in the Interaction of Salivary Glycoproteins with Streptococcus sanguis and Streptococcus mutans , Infect Immun19:107-115, 1978.
6.
Lowry, O.H. ; Roberts, N.R.; Leiner, K.Y.; Wu, M.L.; and Farr, A.L.: The Quantitative Histochemistry of the Brain. I. Chemical Methods, J Biol Chem207:1-17, 1954.
7.
Hudson, B.G. and Spiro, R.G.: Studies of the Native and Reduced and Alkylated Renal Glomerular Basement Membrane, J Biol Chem247:4229-4238, 1972 .
8.
Fairbanks, G. ; Steck, T.L.; and Wallach, D.F.H.: Electrophoretic Analysis of the Major Polypeptides of the Human Erythrocyte Membrane, Biochemistry10:2606-2617, 1971 .
9.
Herzberg, M.C. ; Levine, M.J.; and Tabak, L.A.: Purification and Characterization of Monkey Salivary Mucin, J Biol Chem254:1487-1494, 1979.
10.
Chen, P.S. JR.; Toribara, T.Y.; and Warner, H.: Micro-determination of Phosphorus, Anal Chem28:1756-1758, 1956.
11.
Kleinberg, I.; Ellison, S.A.; and Mandel, I.D.: Saliva and Dental Caries. In: Microbial Abstracts (Sp. Supp.) , New York and London: Information Retrieval, Inc., 1979.
12.
Juriaanse, A.C. and Booij, M.: Isolation and Partial Characterisation of Three Acidic Proteins from Human Submandibular Saliva, Arch Oral Biol24:621-625, 1979.
13.
Juriaanse, A.C. and Booij, M.: Isolation and Characterisation of the Main Neutral Protein from Human Submandibular Saliva, Arch Oral Biol24:825-828, 1979.
14.
Boat, T.F.; Wiesman, U.N.; and Pallavicini, J.C. : Purification and Properties of the Calcium-precipitable Protein in Submaxillary Saliva of Normal and Cystic Fibrosis Subjects, Pediatr Res8:531-539, 1974.
15.
Mayo, J.W. and Carlson, D.M.: Protein Composition of Human Submandibular Secretions, Arch Biochem Biophys161:134-145, 1974.
16.
Mayo, J.W. and Carlson, D.M.: Isolation and Properties of Four a-Amylase Isozymes from Human Submandibular Saliva, Arch Biochem Biophys163:498-506, 1974.
