Extracts of inflamed human gingival tissue converted exogenous [14C] -arachidonic acid mainly to a non-prostaglandin metabolite. This metabolite was identified as 12-hydroxyeicosatetraenoic acid (12-HETE) by gas-liquid chromatography-mass-spectometry. Endogenous arachidonic acid was also converted to 12-HETE. Significant formation of PGF2α , PGD2, PGI2, Thromboxane B, or 12-HHT could not be detected, but, in occasional experiments, significant conversion of arachidonic acid to PGE2 was found.
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