Instrumented root canals of extracted human teeth were inoculated with known pulpal bacterial isolates. The inoculated teeth were immersed in the appropriate culture media and incubated at 37° C for varying periods. Streptococci multiplied in the root canals and invaded the radicular dentinal tubules. The extent of bacterial invasion was time-dependent. This experimental model may be useful in investigating the effect of intra-canal medicaments on microorganisms lodged in the pulpal dentin wall.
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