Tritiated proline was administered as protein marker to domestic cats during the development of their permanent dentition (age 11-18 wk). At 30, 43, 56, and 75 wk, the amount of tritium in the mature dentin was determined by quantitative autoradiography. At 43, 56, and 75 wk the residual amount of label was 92, 92, and 77%, respectively, of that at 30 wk. The metabolism of dentinal collagen (with possibly a recycling of proline) and the metabolism of non-collagenous proline-containing proteins are suggested as factors that may contribute to the decrease of tritium label.
Get full access to this article
View all access options for this article.
References
1.
Borggreven, J.M.P.M.; Hoppenbrouwers , P.M.M.; and Gorissen R.: Radiochemical Determination of the Metabolic Activity of Collagen in Mature Dentin, J Dent Res58 :2120-2124, 1979 .
2.
Carneiro, R. and LeBlond, C.P.: Role of Osteoblasts and Odontoblasts in Secreting the Collagen of Bone and Dentin, as Shown by Autoradiography in Mice Given Tritium-labeled Glycine, Exp Cell Res18:291-300, 1959.
3.
Carneiro, R. and LeBlond, C.P.: Stability of Collagenase Treatment for the Radioautographic Identification of Newly Synthesized Collagen Labeled with 3H-glycine or 3H-proline, J Histochem Cytochem14:334-345, 1965.
4.
Anderson, A.A. : The Protein Matrices of the Teeth and Periodontium in Hamsters: A Tritiated Proline Study, J Dent Res46:67-78, 1967.
5.
Young, R.W. and Greulich, R.C.: Distinctive Autoradiographic Patterns of Glycine Incorporation in Rat Enamel and Dentin Matrices, Arch Oral Biol8:509-521, 1963.
6.
Rogers, A.W. : Techniques of Autoradiography, London: Elsevier, 1973.
7.
Harkness, R.D. : Biological Functions of Collagen, Biol Rev36:399-463, 1961.
8.
Eastoe, J.E. : Chemical Organization of the Organic Matrix of Dentin. In: Structural and Chemical Organization of Teeth, Vol. 2, Miles, A.E.W., Ed., New York: Academic Press, 1967, p. 300.
9.
Jackson, S.H. and Heininger, J.A.: Proline Recycling During Collagen Metabolism as Determined by Concurrent 1802 and 3H-labeling, Biochim Biophys Acta381:359-367, 1975.
10.
Holland, G.R. : The Dentinal Tubule and Odontoblast Process in the Cat, J Anat120:169-177, 1975.
11.
Holland, G.R. : The Extent of the Odontoblast Process in the Cat, J Anat121:133-149, 1976.
12.
Holland, G.R. : An Ultrastructural Survey of Cat Dentinal Tubules , J Anat122:1-13, 1976.
