Storage of Human Periodontal Ligament Cells in a Combination of Different Media

Abstract

Different types of storage media have been studied for use in the replantation of exarticulated teeth. It was found that human periodontal ligament cells survived well in milk. Fifty percent of the cells were viable after 12 hours' storage in milk, while no cells were viable after three hours' storage in saliva. Brief storage in saliva followed by subsequent storage in milk was better than storage in saliva only.

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References

Cvek, M. ; Granath, L. ; and Hollender, L. : Treatment of Non-vital Permanent Incisors with Calcium Hydroxide, Odontol Revy 25:43-56, 1974.

Oswald, R.J. ; Harrington, G.W. ; and Van Hassel , H.J. : A Post-replantation Evaluation of Air-dried and Saliva-stored Avulsed Teeth, J Endo 6:546-551, 1980.

Blomlof, L. and Otteskog, P. : Viability of Human Periodontal Ligament Cells after Storage in Milk or Saliva , Scand J Dent Res 88:436-440, 1980.

Blomlof, L. ; Lindskog, S. ; Hedström, K.-G. ; and Hammarström, L. : Vitality of Periodontal Ligament Cells after Storage of Monkey Teeth in Milk or Saliva, Scand J Dent Res 88:441-445, 1980.

Blomlof, L. ; Lindskog, S. ; and Ham-Marstrom, L. : Periodontal Healing of Exarticulated Monkey Teeth Stored in Milk or Saliva, Scand J Dent Res, In press, 1981. 6. Blomlof, L. ; Otteskog, P. ; and Hammarström , L. : Effect of Storage in Media with Different Ion Strengths and Osmolalities on Human Periodontal Ligament Cells, Scand J Dent Res 89:180-187,1981.

Litwin, J. ; Lundqvist, G. ; and Soder, P.-O. : Studies on Long-term Maintenance of Teeth Viable Associated Cells in vitro , Scand J Dent Res 79:536-539, 1971.

Blomlof, L. and Otteskog, P. : Composition of Human Periodontal Ligament Cells in Tissue Culture, Scand J Dent Res 89: 43-47, 1981.

Mollby, R. ; Thelestam, M. ; and Wad-Strom, T. : Effect of Clostridium perfringens Phospholipase C (Alpha-toxin) on the Human Diploid Fibroblast Membrane, J Membr Biol 16:313-330, 1974.

Ford, J.E. ; Law, B.A. ; Marshall, V.M.E. ; and Reiter, B. : Influence of the Heat Treatment of Human Milk on Some of Its Protective Constituents , J Pediatr 90: 29-35, 1977.