Saliva samples from 120 individuals were cultured by three methods: (a) Dentocult, incubated four d at 37°C or six d at room temperature, (b) SL agar technique, and (c) Snyder test. In a supplementary study with 77 subjects, and comparing only the two former methods, the incubation time with Dentocult at room temperature was prolonged to nine d. The authors also studied whether the number of lactobacilli was influenced by storage time, transport medium, and sampling time of day.
Get full access to this article
View all access options for this article.
References
1.
Fitzgerald, R.J.; Jordan, H.V.; and Archard, H.E.: Dental Caries in Gnotobiotic Rats Infected with a Variety of Lactobacillus acidophilus, Arch Oral Biol11 :473-476,1966.
2.
Rosen, S.; Lenny, W.S.; and O'Malley, J.W.: Dental Caries in Gnotobiotic Rats Inoculated with Lactobacillus casei , J Dent Res47:358-363, 1968.
3.
Fitzgerald, R.J.; Fitzgerald, D.B.; Adams , B.O.; and Duany, L.F.: Cariogenicity of Human Oral Lactobacilli in Hamsters, J Dent Res59:832-837, 1980.
4.
Crossner, C.-G. : The Usefulness of Salivary Lactobacillus Counts in Prediction of Caries Activity. In: Försök till Tidig Diagnos av Kariessjukdomen, Thesis, Umeå University Odontological Dissertations, No. 12, ISSN 0934-7532, 1980 .
5.
Klock, B. and Krasse, B.: A Comparison Between Different Methods for Prediction of Caries Activity , Scand J Dent Res87:129-139, 1979.
6.
Ellen, R.P.: Microbiological Assays for
7.
Dental Caries and Periodontal Disease Susceptibility, Oral Sci Rev8:3-23, 1976.
8.
Larmas, M.: A New Dip-slide Method for the Counting of Salivary Lactobacilli, Proc Finn Dent Soc71:31-35, 1975.
9.
Rogosa, M.; Mitchell, J.A.; and Wise-Man, R.F.: A Selective Medium for the Isolation and Enumeration of Oral Lactobacilli , J Dent Res30:682-689, 1951.
10.
Snyder, M.L. and Arbor, A.: A Simple Colorimetric Method for the Diagnosis of Caries Activity , JADA28:44-49, 1941.
11.
Burnett, G.W. and Scherp, H.W.: Applied Microbiology and Dental Caries. In: Oral Microbiology and Infectious Disease, 3rd ed., Baltimore, MD: The Williams & Wilkins Company, 1968, p. 388.
12.
Appleton, J.L.T.: Bacteriological Infection in Dental Practice, 4th ed., Philadelphia, PA: Lea and Febiger, 1950, p. 415.
13.
Schneyer, L.H. ; Pigman, W.; Hana-Han , L.; and Gilmoure, R.W.: Rate of Flow of Human Parotid, Sublingual, and Submaxillary Secretions During Sleep , J Dent Res35:109-114, 1956.
14.
Lear, C.S.C. ; Flanagan, J.B., Jr.; and Moorrees , C.F.A.: The Frequency of Deglutition in Man, Arch Oral Biol10:83-99, 1965.
15.
Crossner, C.G. and Hagberg, C.: A Clinical and Microbiological Evaluation of the Dentocult® Dip-slide Test, Swed Dent J1: 99-120, 1977.
16.
Snyder, M.L. and Clarke, M.K.: Evaluation of the Colorimetric (Snyder) Test, J Dent Res29:298-303, 1950.
17.
Moller, A.J.R. : Microbiological Examination of Root Canals and Periapical Tissues of Human Teeth, Thesis, Odont Tidskr74: Nos. 5 and 6, 1966.
18.
Bennet, C.A. and Franklin, N.L.: Statistical Analysis in Chemistry and the Chemical Industry, New York, NY: John Wiley & Son, 1954.
19.
Sadler, E. and McQuown, A.L.: Atlas of Medical Mycology, 3rd ed., Baltimore, MD: The Williams and Wilkins Company, 1974.
20.
Davies, G.N. ; King, R.M.; and Coccins, A.A.: The Relation Between Lactobacillus Counts, Snyder Tests, and the Subsequent Incidence of Dental Caries, Arch Oral Biol1:62-73, 1959.
21.
Sims, W.: The Interpretation and Use of Snyder Tests and Lactobacillus Counts , JADA80:1315-1319,1970.
22.
Rogosa, M.: Characters used in the Classification of Lactobacilli, Int J Syst Bacteriol20: 519-533, 1970.
23.
Hammond, B.F. and Williams, N.B.: Studies on Encapsulated Lactobacilli. I. Utilization of Capsular Material by L. casei, Arch Oral Biol9:341-349, 1964.
24.
Hammond, B.F. : Studies on Encapsulated Lactobacilli. III. Human Oral Strains, J Dent Res46:340-346, 1967.
25.
Grubb, R. and Krasse, B.: Sampling Methods for the Determination of the Number of Lactobacilli in the Oral Cavity, Acta Odont Scand12:145-156, 1954.
26.
Jordan, H.V. ; Krasse, B.; and Moller, A.: A Method of Sampling Human Dental Plaque for Certain "Caries-inducing" Streptococci, Arch Oral Biol13:919-927, 1968.
27.
Gastrin, B. ; Kallings, L.O.; and Mar-Cetic , A.: The Survival Time for Different Bacteria in Various Transport Media, Acta Path Microbiol Scand74:371-380, 1968.
