Gingival fluid was collected from children with a gingival index score of 2. Lipids were extracted, purified, and separated by thin-layer nanochromatography and identified. Lysophosphatidylcholines, sphingomyelins, phosphatidyl-cholines-ethanolamines-serines-inositols, polyglycerophosphatides, diphosphatidylglycerols, non-esterified fatty acids, mono- and di-glycerides, and cholesterols were observed. Serum origin of these lipids was possible, but enrichment by salivary bacterial or tissue lipids cannot be eliminated.
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