Hydroxyapatite-treated clarified mixed saliva showed a marked reduction in growth-supportive activity for Streptococcus mutans. Isoelectrophoretic analysis revealed that proteins specifically attacked by S. mutans were among those adsorbed by HA. The proteins desorbed from HA-supported growth of S. mutans at levels comparable to untreated saliva.
Oppenheim, F.G. ; Hay, D.I.; and Franz-Blau , C.: Proline-rich Proteins from Human Parotid Saliva. I. Isolation and Partial Characterization , Biochemistry10:4233-4238, 1971 .
3.
Hay, D.I. and Oppenheim, F.G.: The Isolation from Human Parotid Saliva of a Further Group of Proline-rich Proteins, Arch Oral Biol19:627-632, 1974.
4.
Hay, D.I. : Fractionation of Human Parotid Salivary Proteins and the Isolation of a Histidine-rich Acidic Peptide Which Shows High Affinity for Hydroxyapatite Surfaces, Arch Oral Biol21 :553-558, 1975.
5.
Hay, D.I. : The Isolation from Human Parotid Saliva of a Tyrosine-rich Acidic Peptide Which Exhibits High Affinity for Hydroxyapatite Surfaces, Arch Oral Biol18:1531-1541, 1973.
6.
Hay, D.I. and Schlesinger, D.H. : Human Salivary Statherin: A Peptide Inhibitor of Calcium Phosphate Precipitation. In: Calcium Binding Proteins and Calcium Function, Wasserman, R.H., Corradino, R.A., Carafoli, E., Kretsinger, R.H., MacLennan, D.H., and Siegal, F.L., Eds., Elsevier North-Holland, Inc., 1977, pp. 401-408.
7.
Belcourt, A. : Adsorption of Human Salivary Proteins on Synthetic Hydroxyapatite, Arch Oral Biol21:717-722, 1976. 8. Bennick, A. and Connell, G.E.: Purification and Partial Characterization of Four Proteins from Human Parotid Saliva, Biochem J123:455-464, 1971.
8.
Bennick, A.: Chemical and Physical Characteristics of a Phosphoprotein from Human Parotid Saliva, Biochem J145:557-567, 1975.
9.
Bennick, A. : Chemical and Physical Characterization of a Phosphoprotein, Protein C, from Human Saliva and Comparison with a Related Protein A, Biochem J163:229-239, 1977.
10.
Cowman, R.A. ; Fitzgerald, R.J.; and Schaefer , S.J.: Role of Salivary Factors in the Nitrogen Metabolism of Plaque-forming Oral Streptococci , Proceedings: Microbial Aspects of Dental Caries , Stiles, Loesche, and O'Brien , Eds., Sp. Supp. Microbiology Abstracts, Vol. II, 1976, pp. 465-475.
11.
Cowman, R.A. ; Fitzgerald, R.J.; Perrella , M.M.; and Cornell, A.H.: Human Saliva as a Nitrogen Source for Oral Streptococci, Caries Res11:1-8, 1977.
12.
Cowman, R.A. and Fitzgerald, R.J. : Effects of Oral Streptococci on Electrophoretic Properties of Human Salivary Anionic Proteins, J Dent Res54:298-303, 1975.
13.
Cowman, R.A. ; Schaefer, S.J.; and Fitzgerald , R.J. : Specificity of Utilization of Human Salivary Proteins for Growth by Oral Streptococci , Caries Res13:181-189, 1979.
14.
Cowman, R.A. ; Perrella, M.M.; and Fitzgerald , R.J.: Influence of Incubation Atmosphere on Growth and Amino Acid Requirements of Streptococcus mutans, Appl Microbiol27:86-92, 1974.
15.
Steiner, J.C. and Keller, P.J.: An Electrophoretic Analysis of the Protein Components of Human Parotid Saliva, Arch Oral Biol13:1213-1221, 1968.
16.
Hay, D.I. : The Adsorption of Salivary Proteins by Hydroxyapatite and Enamel, Arch Oral Biol12:937-946, 1967.
17.
Pruitt, K.M. ; Caldwell, R.C.; Jamie-Son , A.D.; and Taylor, R.E.: The Interaction of Salivary Proteins with Tooth Surface, J Dent Res48:818-823, 1969.
18.
Stiefel, D.J. : Characteristics of an in vitro Dental Pellicle , J Dent Res55:66-73, 1976.
19.
Cowman, R.A. ; Schaefer, S.J.; Fitzgerald , R.J.; Rosner, D.; Shklair, I.L.; and Walter, R.G.: Differential Utilization of Proteins in Saliva from Caries-active and Caries-free Subjects as Growth Substrates by Plaque-forming Streptococci, J Dent Res58:2019-2027, 1979.
20.
Cowman, R.A. ; Schaefer, S.J.; Oppenheim , F.G.; and Hay , D.I.: Statherin and the Proline-rich Proteins PRP II and PRP IV as Amino Nitrogen Sources for Plaque-forming Oral Streptococci , J Dent Res58: 2008-2009, 1979 .
21.
Leach, S.A. ; Critchley, P.; Kolendo , A.B.; and Saxton, C.A.,: Salivary Glycoproteins as Components of the Enamel Integuments, Caries Res1:104-111, 1967.
22.
Mayhall, C.W. : Concerning the Composition and Source of the Acquired Enamel Pellicle of Human Teeth, Arch Oral Biol15: 1327-1341,1970.
23.
Sönju, T. and Rölla, G.: Chemical Analysis of the Acquired Pellicle Formed in Two Hours on Cleaned Human Teeth in vitro, Caries Res7:30-38, 1973.