Ultrastructural morphology of 16 salivary calculi was studied by means of transmission and scanning electron microscopy. The external surface was mostly globular or coarse, and on high magnifications the features could be divided into four main groups: a) amorphic calcified deposits covering extensive areas, b) other areas covered with crystals in a variety of arrangements, c) heavy accumulations of calcified rod-like and filamentous-like microorganisms, and d) platelet crystals in juxtaposition to calcified microorganisms in several areas. In most calculi the split area was found to be laminated. It is suggested that microorganisms have an important role in the formation and growth of salivary calculi.
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References
1.
Rauch, S. and Gorlin, R.: Thoma's Oral Pathology, 6th ed., Gorlin, R.J. and Gold-man, H.M., Eds., St. Louis: C.V. Mosby Co., 1970, pp. 997-1002.
2.
Rust, T.A. and Messerly, C.D.: Oddities of Salivary Calculi, Oral Surg28:862-&65, 1969.
3.
Allan, J.H.; Finch, L.D.; and Chippen-Dale, I. : Sialolit hia sis of the Minor Salivary Glands , Oral Surg27:780-785, 1969.
4.
Blatt, I.M.; Denning, R.M.; Zumberge, J.H.; and Maxwell, J.H.: Studies in Sialolithiasis. 1. The Structure and Mineralogical Composition of Salivary Gland Calculi, Ann Otol67:595-617, 1958.
5.
Harrill, J.A. ; King, J.S.; and Boyce, W.H.: Structure and Composition of Salivary Calcul , Laryngoscope69:481-492, 1959.
6.
Anneroth, G. ; Eneroth, C.M.; and Isacsson , G.: Morphology of Salivary Calculi. The Distribution of the Inorganic Component, J Oral Path4:257-265, 1975.
7.
Von Derthold, M.; Schneider, M.J.; and Tscharnke , J.: Struktur und Chemischer Aufbau der Speichelsteine, Dtsch Zahnarztl Z25:743-747, 1970.
8.
Jensen, A.T. and Dano, M.: X-ray Crystaltographic Examination of Calculi from Salivary Glands, J Dent Res31:620-626, 1952.
9.
Vahl, J.; Pfefferkorn, G.; and Möh-Ling, H.J. : Sublichtmikroskopische Untersuchungen am Menschlichen Speichelstein, Dtsch Zahnarztl Z23:39-44, 1968.
10.
Anneroth, G. ; Eneroth, C.M.; and Isacsson , G.: Crystalline Structure of Salivary Calculi A Microradiographic and Microdiffractometric Study, J Oral Path4: 266-272,1975.
11.
Klebs, E.: Beitrage zur Kenntniss der Pathogenen Schistomyceten, Schmiedeberg's Archiv fuer Experirxtentalle Pathologie und Pharmakologie5 :350-377, 1876.
12.
Naeslund, C. : Studien über Speichelsteinbiidung, Acta Peth et Microbiol Scand2: 244-276, 1925.
13.
Naeslund, C. : Fortgesetzte Untersuchungen uber die Speichelsteinbildung unter Besonderer Berucksichtigung ihres Verhaltmsses zur Entstehung Primarer Speicheldrusenaktinomykose, Acta Path et Microbiol Scand6:78-96, 1929.
14.
Söderlund, G.: Die Speichelsteinkrankheit (Sialolithiasis) und ihr Verhalten zu der Primaren und Duktogenen Speicheidrusenaktinomykose , Acta Chir Scand63: Suppl. No. 9, 1927.
Risak, E.: Zur Klinik und Pathologie der Speichelsteinkrankheit, Dtsch Zeitschr fuir Chir219:206-220, 1929.
18.
Risak, E.: Zur Frage der Speichelsteinkrankheit, Zentralblatt für Chir39:2464, 1929.
19.
Anneroth, G. ; Eneroth, C.M.; Isacsson , G.; and Lundquist, P.G.: Ultra-structure of Salivary Calculi, Scand J Dent Res96:182-192, 1978.
20.
Höhling, H.J. ; Pfefferkorn, G.; Radicke , J.; and Vahl, J.: Elektronenmikroskopische Untersuchungen zur Organischen Matrix und Kristallbildung in Menschlichen Speichelteinen, Dtsch Zehmarztl Z24:663-670, 1969.
21.
Higashi, S. ; Fukuda, K.; Tago , A.; Onoe, A.; Takahashi, H.; Imaizumi, I.; Ichikawa, T.; and Mita, H.: Preparation and Observation of Serial-decalcified Sections of Salivary Calculus, Shikwa Gakuho72:230-242, 1972.
22.
Ennever, J. : Intracellular Calcification by Oral Filamentous Microorganisms, J Periodont31:304-307, 1960.
23.
Ennever, J.: Microbiologic Calcification, Ann NYAcad Sci109:4-13, 1963.
24.
Ennever, J. and Creamer, H.: Microbiologic Calcification: Bone Mineral and Baeteria, Calcif Tiss Res1:87-93,1967. Ennever, J.; Streckfuss, J.L.; and
25.
Takazoe, I.: Calcification of Bacillary and Streptococcal Variants of Bacterionema matruchotii , J Dent Res52:305-308, 1973.
26.
Lie, T. and Selvig, K.A.: Calcification of Oral Bacteria: An Ultrastructural Study of Two Strains of Bacterionema matruchotii, Scand J Dent Res82:8-18, 1974.
27.
Lie, T. and Selvig, K.A.: Effect of Salivary Proteins on Calcification of Oral Bacteria, Scand J Dent Res82:135-143, 1974.
28.
Rizzo, A.A.; Martin, G.R.; Scott, D.B.; and Mergenhagen, S.E. : Mineralization of Bacteria, Science135:439-441, 1962.
29.
Rizzo, A.A. ; Scott, D.B.; and Bladen, H.A.: Calcification of Oral Bacteria, Ann NY Acad Sci109:14-22, 1963.
30.
Takazoe, I. ; Kurahashi, Y.; and Takuma, S.: Electran Microscopy of Intracellular Mineralization of Oral Filamentous Microorganisms in vitro , J Dent Res42: 681-695,1963.
31.
Gonzales, F. and Sognnaes, R.F.: Electronmicroscopy of Dental Calculus, Science131:156-158, 1960.
32.
Zander, H.A. ; Hazen, S.P.; and Scott, D.B.: Mineralization of Dental Calculus. Proc Soc Exp Biol (NY)103:257-260, 1960.
33.
Schroeder, H.E.: Formation and Inhibition of Dental Calculus, Berne : Hans Huber, 1969, pp. 94-122.
34.
Lustmann, J. ; Lewin-Epstein, J.; and Shteyer, A.: Scanning Electron Microscopy of Dental Calculus , Calcif Tiss Res21: 47-55,1976.
