Biosynthesis of Prostaglandin E2 and F2a in Gingiva of Patients with Chronic Periodontitis

Abstract

The biosynthesis of radioactive prostaglandin E2 (PGE₂) and F _2α from (] .14C) arachidonic acid has been demonstrated in the microsomal fraction of gingiva of patients with chronic periodontitis in vitro. ¹⁴C corresponding to unmetabolized arachidonic acid in three separate incubations was 83, 66, and 83%, and that corresponding to both PGE₂ and PGF_2α was 8, 22, and 9% of the total radioactivity recovered after chromatography. The ratios of PGE₂ to PGF _2α were found to be 6:1, 6:1, and 3:1, respectively.

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