Palatal shelves from 14-day-old embryonic mice were exposed to a 27.12 MHz pulsed non-ionizing radiofrequency (Diapulse) for 20 min followed by 24-hour organ culture in nutrient agar. Diapulse-treated palatal shelves showed induction of cartilage within the mesenchymal compartment and loss of the overlying epithelium in contrast to controls which were free of cartilage. The results are thought to be independent of thermal changes and may be due to calcium flux within the tissue.
Get full access to this article
View all access options for this article.
References
1.
Ginsberg, A.J. : Ultrashort Radiowaves as a Therapeutic Agent , Med Record140 :651-653,1934.
2.
Cameron, B.M. : Experimental Acceleration of Wound Healing , Am J Orthopedics3 :336-343, 1961.
3.
Caney, W.H.: Burns and Their Treatment with Diapulse, Letter to the Diapulse Corporation , November 25, 1964.
4.
Nadasdi, M.: Inhibition of Experimental Arthritis by Athermic Pulsating Short Wave in Rats , Orthopedics2:105-107, 1960.
5.
Aronofski, D.H. : Reduction of Dental Post-surgical Symptoms Using Nonthermal Pulsed High-peak Power Electromagnetic Energy, Oral Surg, Oral Med, Oral Path32: 688-696,1971.
6.
Tyler, M.S. and Koch, W.E.: In vitro Development of Palatal Tissues from Embryonic Mice. I. Differentiation of the Secondary Palate from 12-day Mouse Embryos, Anat Rec182:297-304, 1975.
7.
Callas, G. and Walker, B.E.: Palate Morphogenesis in Mouse Embryos after X-irradiation, Anat Rec145:61-71, 1963.
8.
Robbins, S.L. : Pathology, 3rd ed., Philadelphia : W. B. Saunders, 1967, p. 81.
9.
West, B. and Regelson, W.: Biological Activity Associated with Pulsed High Frequency Radiation (27.12 MHz), In Biological Effects of Electromagnetic Waves . C. C. Johnson and M. L. Shore, Eds., Washington: U.S. Department of Health, Education, and Welfare, 1976 , pp. 260-280.
10.
Smith, S.D.; Thomas, C.L.; and Frasch, S.F.: Lanthanum Inhibition of Electrically
11.
induced Differentiation in Frog Erythrocytes, In IV International Symposium on Bioelectrochemistry. A. Pilla , Ed., Woods Hole, 1977, p. 60.
12.
Moran, D.: Calcium-mediated Morphogenetic Movements During Neuralation, In IV. International Symposium on Bioelectrochemistry. A Pilla, Ed., Woods Hole, 1977, p. 63.
13.
Bassett, C.A.L.: Electrical Augmentation of Bone Growth and Repair, In IV International Symposium on Bioelectrochemistry. A. Pilla, Ed., Woods Hole, 1977, p. 64.
14.
Becker, R.O.: Current Status on the Electrical Stimulation of Bone Growth, In IV International Symposium on Bioelectrochemistry. A. Pilla, Ed., Woods Hole, 1977,p.161.
15.
Sisken, B.: Comparison of the Effects of Minute Levels of Direct Electrical Current and Nerve Growth Factor on Embryonic Sensory Ganglia in vitro, In IV International Symposium on Bioelectrochemistry. A Pilla, Ed., Woods Hole, 1977, p. 57.
16.
Pilla, A.A. : Electrochemical Information Transfer at Living Cell Membranes, Ann NY Acad Sci238:149-170, 1974.
17.
Pilla, A.A.: An Interfacial Electrochemical Approach to the Control of Cellular Behavior , In IV International Symposium on Bioelectrochemistry . A. Pilla, Ed., Woods Hole, 1977, p. 57.
18.
Pilla, A.A. and Margules, G.S.: Dynamic Interfacial Phenomena at Living Cell Membranes : Application to the Toad Urinary Bladder Membrane System, J Electrochem Soc124:1697-1706, 1977 .
19.
Riley, V.: Intrinsic Effects of Electromagnetic Radiation on Cancer Cells, Proc Am Assoc for Cancer Res20:533, 1979.
20.
Lary, J.M.; Conover, D.L.; Foley, E.D.; and Hanser, P.L.: Teratogenicity of 27.12 MHz Radiofrequency Radiation in Rats, Teratology19:36A, 1979.
