The periodontal ligaments from traumatized deciduous teeth, while undergoing rapid resorption, were analyzed biochemically for mucopolysaccharidase activity and for total glycosaminoglycan content of dentin and cementum. Enzyme activity was present only in resorbing teeth. A concomitant 65 percent decrease in glycosaminoglycans from these teeth occurred as well.
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References
1.
Finn, S.B.: Clinical Pedodontics, 4th Ed. Philadelphia: W. B. Saunders Co., 173, p. 50.
2.
Munemoto, K. ; Iwayama, Y.; Yoshida , M.; Sera, M.; Aono, M.; and Yokomizo, I.: Isolation and Characterization of Acid Mucopolysaccharides of Bovine Periodontal Membrane , Archs Oral Biol15:369-382, 1970.
3.
Engfeldt, B. and Hjerpe, A.: Glycosaminoglycans of Dentine and Predentine , Calc Tiss Res10:152-159, 1972.
4.
Sicher, H. and Bhaskar, S.N.: Orban's Oral Histology and Embryology, 7th Ed. St. Louis: C. V. Mosby Co. , 1972, pp. 160, 320.
5.
Clark, R.D.; Smith, J.G.; and Davidson, E.A.: Hexosamine and Acid Glycosaminoglycans in Human Teeth, Biochim Biophys Acta101:267-272, 1965.
6.
Alexander, S.A. and Swerdloff, M.: Mucopolysaccharidase Activity During Human Deciduous Root Resorption: A Preliminary Report, Archs Oral Biol, in press, 1979.
7.
Alexander, S.A. : Unpublished data.
8.
Polansky, J.R. ; Toole, B.P.; and Gross, J.: Brain Hyaluronidase: Changes in Activity During Chick Development, Science183: 862-864, 1974.
9.
Muir, J.: The Structure and Metabolism of Mucopolysaccharides (Glycosaminoglycans) and the Problem of the Mucopolysaccharidases, Am J Med47:673-690, 1969.
10.
Reissig, J.L. ; Strominger, J.L.; and Leloir, L.F.: A Modified Colorimetric Method for the Estimation of N-acetylamino Sugars, J Biol Chem217:959-966, 1955.
11.
Margolis, R.B. ; Margolis, R.K.; San-Tella , R.; and Atherton, D.M.: The Hyaluronidase of Brain, J Neurochem19: 2325-2332,1972.
12.
Lowry, O.H. ; Rosenbrough, N.J.; Farr , A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193:265-275, 1951.
13.
Bertolami, C.N. and Donoff, R.B.: Hyaluronidase Activity During Open Wound Healing in Rabbits: A Preliminary Report, J Surg Res25:256-259, 1978.
14.
Bernick, S. ; Rabinowitch, B.Z.; and Rutherford , R.L.: Microscopic Studies of the Teeth of a Six-year-old Boy. II. Tooth Absorption, Anat Rec105:249-258, 1949.
15.
Obersztyn, A. : Experimental Investigation of Factors Causing Resorption of Deciduous Teeth, J Dent Res42:660-674, 1963.
16.
Soskolne, W.A. and Bimstein, E.: A Histomorphological Study of the Shedding Process of Human Deciduous Teeth at Various Chronological Stages , Archs Oral Biol22:331-335,1977.
17.
Weatherell, J.A. and Hargreaves , J.A.: Effect of Resorption on the Fluoride Content of Human Deciduous Teeth, Archs Oral Biol11 :749-756, 1966.
18.
Goggins, J.F. ; Fullmer, H.M.; and Steffek, A.J.: Hyaluronidase Activity of Human Gingiva , Arch Path85:272-274, 1968.
19.
Aronson, N.N. and Davidson, E.A.: Lysosomal Hyaluronidase from Rat Liver. II. Properties, J Biol Chem242:441-444, 1967.
20.
Wood, G.C.: The Precipitation of Collagen Fibers from Solution, Int Rev Connect Tiss Res2:1-29, 1964.
21.
Toole, B.P. and Lowther, D.A.: Precipitation of Collagen Fibrils in vitro by Protein Polysaccharides, Biochem Biophys Res Commun29:515-520, 1967.
22.
Kawamoto, T. and Nagai, Y.: Developmental Changes in Glycosaminoglycans, Collagen and Collagenase Activity in Embryonic Chick Skin, Biochim Biophys Acta437: 190-199, 1976.
23.
Hascall, V.C. and Heinegard, D.: The Structure of Cartilage Proteoglycans, In: Extracellular Matrix Influences on Gene Expression (Slavkin, H. C. and Greulich, R. C., Editors). New York: Academic Press, 1975, p. 423.
24.
Rosenberg, L.; Margolis, R.; Wolf-Enstein-Todel, C.; Pal, S.; and Stri-Der, W.: Organization of Extracellular Matrix in Bovine Articular Cartilages , In: Extracellular Matrix Influences on Gene Expression (Slavkin, H. C. and Greulich, R. C., Editors). New York: Academic Press, 1975,p.415.
25.
Woodhead-Gallowy, J. and Hukins, D.W.L.: Molecular Biology of Cartilage , Endeavor35:73-78, 1975.
26.
Woolley, D.E. and Evanson, J.M.: Effect of Cartilage Proteoglycans on Human Collagenase Activities, Biochim Biophys Acta497:144-150,1977.
27.
Linares, H.A. and Larsen, D.L.: Proteoglycans and Collagenase in Hypertrophic Scar Formation, Plast & Reconstr Surg62:589-593, 1978.
28.
Morita, H.; Yamashiya, H.; Shimizu, M.; and Sasaki, S.: The Collagenolytic Activity During Root Resorption of Bovine Deciduous Teeth , Archs Oral Biol15 :503-508, 1970.
29.
Iijima, K.; Kishi, J.; Fuyamada, H.; Nagatsu, T.; and Hayakawa, T.: Dental Sac Collagenase: Demonstration and Initial Characterization , J Dent Res57: 724,1978.
