Mononuclear cells isolated from peripheral whole blood were fractionated by erythrocyte-rosetting into T cells and non-T cell subpopulations which were tested for collagenase-producing capacity. Mononuclear cells and purified T lymphocytes, as well as non-T (B) lymphocytes, produced collagenase during serum-free culture. Removal of the macrophages from the non-T cells did not affect collagenase production by lymphocytes.
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References
1.
Schroeder, H.E. ; Munzel-Pedraz-Zoli , S.; and Page, R.: Correlated Morphometric and Biochemical Analysis of Gingival Tissue in Early Chronic Gingivitis in Man, Arch Oral Biol18:899-923, 1973.
2.
Schroeder, H.E. ; Graf DEBeer, M.; and Attstrom, R.: Initial Gingivitis in Dogs, J Periodont Res110:128-142, 1975.
3.
Flieder, D.E. ; Sun, C.N.; and Schnei-Der , B.C.: Chemistry of Normal and Inflamed Human Gingival Tissues, Periodontics4:302-307,1966.
4.
Dresden, M.H. : Evidence for the Role of Collagenase in Collagen Metabolism, Nature (New Biol)231:55-57, 1971.
5.
Mailman, M.L. : Collagenase and Collagen Degradation: Presence of TCA Fragments In Vivo, AADR Prog & Abst56:No. 125, 1978.
6.
Sakai, T. and Gross, J.: Some Properties of the Products of Reaction of Tadpole Collagenase with Collagen , Biochem6 :518-528,1967.
7.
Tanzer, M.L.: Biochemistry of Collagen (Edited by G.N. Ramachandran and E.H. Reddi), New York/London: Plenum, pp. 137-162, 1976.
8.
Burleigh, M.C.: Degradation of Collagen by Non-Specific Proteinases. In Proteinases in Mammalian Cells and Tissues (Edited by A. J. Barrett), New York: North-Holland , 285-309, 1977.
9.
Beutner, E.; Thrifthauser, C.; and Hanzen, S.P.; Beutner, E.H.; and Thrifthauser, C. : Collagenolytic Activity tal Disease, Proc Soc Exp Biol Med121 :1082-1085,1966.
10.
Hanzen, S.P.; Beutner, E.H.; and Thrifthauser, C. : Collagenolytic Activity of Gingival Tissue Affected with Periodontal Disease, J Periodont39:45, 1968.
11.
Fullmer, H.M.: Metabolic Hydrolysis of Collagen. In Metabolic Conjugation and Metabolic Hydrolysis , Vol 2, New York, London: Academic Press, 301-343, 1970.
12.
Golub, L.M. ; Siegel, K.; Ramamurthy, N.S.; and Mandel, I.D.: Some Characteristics of Collagenase Activity in Gingival Crevicular Fluid and Its Relationship to Gingival Disease in Humans , J Dent Res55: 1049-1057, 1976 .
13.
Fullmer, H.M. and Gibson, W.: Collagenolytic Activity in Gingiva of Man , Nature209:728-729,1966.
14.
Fullmer, H.M. ; Gibson, W.A.; Lazurus, G.S.; Bladen, H.A.; and Whedon, K.A.: The Origin of Collagenase in Periodontal Tissues of Man, J Dent Res48:646-651, 1969.
15.
Lazurus, G.S. ; Brown, R.S.; Daniels, J.R.; and Fullmer, H.M.: Human Granulocyte Collagenase, Science159:1483-1484, 1968.
16.
Senior, R.M. ; Bielefeld, D.R.; and Jeffery, J.J.: Collagenase Activity in Alveolar Macrophages , Clin Res20:88, 1972.
17.
Wahl, L.M.; Wahl, S.M.; Mergenha-Gen, S.E.; and Martin, G.R.: Collagenase Production by Endotoxin-Activated Macrophages, Proc Nat Acad Sci USA71 :3598-3601, 1974 .
18.
Page, R.C. and Schroeder, H.E.: Pathogenesis of Inflammatory Periodontal Disease, Lab Invest33:235-249, 1976.
19.
Mackler, B.F. ; Frostad, K.D.; Robertson, P.B.; and Levy, B.M.: Immunological Mechanisms in Periodontal Disease, J Periodont Res12:37-45, 1977.
20.
Mackler, B.F. ; Waldrop, T.C.; Schur, P.; Robertson, P.B.; and Levy, B.M.: IgG Subclass in Human Periodontal Disease. I. Distribution and Incidence of IgG Subclass Bearing Lymphocytes and Plasma Cells, J Periodont Res13:109-119, 1978.
21.
Mackler, B.F. ; Faner, R.M.; Schur, P.; Wright, T.E. III; and Levy , B.M.: IgG Subclass in Human Periodontal Disease. II. Cytophilic and Membrane IgG Subclass Immunoglobulins , J Periodont Res13 :433-444, 1978.
22.
Mackler, B.F. ; O'Neill, P.A.; and Meistrich , M.: T Lymphocyte Induction of Non-T Cell-Mediated Nonspecific Cytotoxicity, Europ J Immunol1:56-61, 1977.
23.
Golstein, P. and Blomgren, H.: Further Evidence of Autonomy of T-Cells Mediating Specific In Vitro Cytotoxicity: Efficiency of Very Small Amount of Highly Purified T-Cells, Immunol9:127-141, 1973.
24.
Yam, L.T. ; Li, C.Y.; and Crosby, W.H.: Cytochemical Identification of Monocytes and Granulocytes, Am J Clin Pathol53: 283-289, 1971.
25.
McJunkin, F.A. : Peroxydase Staining with Benzidine in Paraffin Sections of Human Tissues, Anat Rec24:67-77, 1969.
Meistrich, M.L. and Prescott, D.M. (ed): Separation of Spermatogenic Cells and Nuclei From Rodent Testes, New York: Academic Press, 1976.
28.
Meistrich, M.L.; Nell, L.J.; Richie, E.S.; Mackler, B.F.; and O'Neill , P.A.: Rapid Separation of Viable Lymphoid Cells by Centrifugal Elutraitor, Fed Am Soc Exptl Biol Abstracts, 63rd Annual Meeting , 1979.
29.
Gross, J.: Studies on the Formation of Collagen. 1. Properties and Fractionation of Neutral Salt Extracts of the Normal Guinea Pig Connective Tissue, J Exp Med107 :247-263,1958.
30.
Fullmer, H.M. ; Taylor, R.E.; and Guthrie, R.W.: Human Gingival Collagenase : Purification, Molecular Weight and Inhibition Studies, J Dent Res51:349-355, 1972.
31.
Nagai, Y.; Gross, J.; and Piez, K.A.: Disc Electrophoresis of Collagen Components, Ann N.Y. Acad Sci121:484-500, 1964.
32.
Lowry, O.H. ; Rosenbrough, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193:265-275, 1951.
33.
Hook, R.M.; Hook, C.W.; and Brown, S.I.: Fibroblast Collagenase: Partial Purification and Characterization, Invest Ophthalmol12:771-776,1974.
34.
Bauer, E.A. ; Strickland, G.P.; Jeffery, J.J.; and Eisen, A.Z.: Collagenase Production by Human Skin Fibroblasts , Biochem Biophys Res Comm64:232-240, 1975.
35.
Rose, G.G. and Robertson, P.B.: Collagenolysis by Human Gingival Fibroblast Cell Lines, J Dent Res56:416-423, 1977.
36.
Yajima, T. and Rose, G.G.: Phagocytosis of Collagen by Human Gingival Fibroblasts In Vitro, J Dent Res56:1271-1277, 1977 .
37.
Ten Cate, A.R. ; Morphological Studies of Fibrocytes in Connective Tissue Undergoing Remodeling, J Anat112:401-414, 1972.
38.
Listgarten, M.A. : Intracellular Collagen Fibrils in the Periodontal Ligament of Mouse, Rat, Hamster, Guinea Pig and Rabbit, J Periodont Res8:335-342, 1973.
39.
Eley, B.M.; and Harrison, J.D.: Intracellular Collagen Fibrils in the Periodontal Ligament of Man, J Periodont Res10 :168-170,1975.
40.
Svoboda, E.L.A. ; Brunette, D.M.; and Melcher, A.H.: A Novel Hypothesis of the Mechanism of Collagen Phagocytosis by Periodontal Ligament Cells In Vitro, J Dent Res57 (Special Issue A), 224, 1978.
