Calcium, but not magnesium or strontium ions, specifically induce the precipitation of dentin phosphoprotein, which is reversibly solubilized by EDTA. This finding is very useful in purifying dentin phosphoprotein of either a free or matrix-bound type. Bovine dentin phosphoproteins, thus isolated, contain a minimal amount of contaminant protein, unlike previous preparations.
Get full access to this article
View all access options for this article.
References
1.
Veis, A. and Perry, A.: The Phosphoprotein of the Dentin Matrix, Biochemistry8:2409-2419,1967.
2.
Veis, A.; Spector, A.R.; and Zamo-Sianyk, H.: The Isolation of an EDTAsoluble Phosphoprotein from Mineralizing Bovine Dentin , Biochem Biophys Acta257: 404-413, 1972.
3.
Lee, S.L. ; Veis, A.; and Glonek, T.: Dentin Phosphoprotein: An Extracellular Calcium-binding Protein, Biochemistry16 :2971-2979, 1977.
4.
Nawrot, C.F. ; Chambell, D.J.; Schroeder, J.K.; and Valkenberg , M.V.: Dental Phosphoprotein-induced Formation of Hydroxyapatite During in vitro Synthesis of Amorphous Calcium Phosphate, Biochemistry15:3445-3449, 1976 .
5.
Weinstock, M. and Leblond, C.P.: Radioautographic Visualization of the Deposition of a Phosphoprotein at the Mineralization Front in the Dentin of the Rat Incisor , J Cell Biol56:838-845, 1973.
6.
Kuboki, Y.; Shimokawa, H.; Ono, T.; and Sasaki, S.: Detection of Collagen Degradation Products in Bone, Calcif Tiss Res12:303-312,1973.
7.
Kuboki, Y.; Tanzer, M.L.; and Mechanic, G.L.: Isolation of Polypeptides Containing the Intermolecular Cross-link of Dihydroxylysinonorleucine from Dentin Collagen, Arch Biochem Biophys158 :106-115,1973.
8.
Colowick, S.P. and Womack, F.C.: Binding of Diffusible Molecules by Macromolecules : Rapid Measurement by Rate of Dialysis, J Biol Chem244:774-779, 1969.
9.
Reed, K.C.: Binding of Calcium by Cellulose Membranes and Sephadex, Biochem Biophys Res Commun50:1136-1142,1973.
10.
Scatchard, G.: The Attraction of Proteins for Small Molecules and Ions, Ann N. Y. Acad Sci51:660-672, 1949.
11.
Munksgaard, E.C.; Butler, W.T.; and Richardson , W.S.: Phosphoprotein from Dentin. New Approaches to Achieve and Assess Purity, Preparat Biochem7:321-331, 1977.
12.
Dickson, I.R. ; Dimusio, M.T.; Volpin, D; Anathanarayanan, S.; and Vies, A.: The Extraction of Phosphoproteins from Bovine Dentin, Calcif Tiss Res19:51-61, 1975.
13.
Grizzuti, K. and Perlmann, G.E.: Binding of Magnesium and Calcium Ions to the Phosphoglycoprotein Phosvitin , Biochemistry12:4399-4403, 1973 .
14.
Bingham, E.W. ; Farrell, M.F., Jr; and Carroll , R.J.: Properties of Dephosphorylated αs1-casein. Precipitation by Calcium Ions and Micelle Formation, Biochemistry11:2450-2454,1972.
15.
Kuboki, Y.; Shimokawa, H.; Kato, S.Sasaki, S.; Oguchi, H.; and Mechanic, G.L.: The Location of Intermolecular Cross-links in Bone Collagen, Bone Metabolism8:315-323, 1975, in Japanese.
