Indirect immunofluorescent labeling shows a sequential increase in detectable epithelial cell actin and myosin during wound repair migration and maturation. While actin shows a substantial increase between pre-wound and migration levels, the relative detectable myosin is omnipresent at apparently high levels, and it increases only slightly during active migration.
Get full access to this article
View all access options for this article.
References
1.
Coons, A.H.; Creech, H.J.; and Jones, R.N.: Immunological Properties of an Antibody Containing a Fluorescent Group, Proc Soc Exp Biol Med47:200, 1941.
2.
Fujiwara, K. and Pollard, T.D.: Fluorescent Antibody Localization of Myosin in the Cytoplasm, Cleavage Furrow, and Mitotic Spindle of Human Cells, J Cell Biol71:848-875,1976.
3.
Stahl, S.S.: Healing Gingival Surgery in Normal and Systemically Stressed Young Adult Male Rats, J Periodontol32:63-73, 1961.
4.
Ramfjord, S.P. and Costich, E.R.: Healing After Simple Gingivectomy, J Perio34:401-415, 1963.
5.
Mittleman, H.R.; Toro, P.D.; Sicher, H.; and Wentz, F.H.: Healing in Attached Human Gingiva, Periodontics2:106-114, 1964.
6.
Innes, P.H.: An Electronmicroscopic Study of the Regeneration of Gingival Epithelium Following Gingivectomy in the Dog, J Perio Res5:196-204, 1970.
7.
Winter, G.D.: Proceedings Brown University Symposium on Biology of Skin, Vol. 5, Montagna, W., and Billingham, R. E., (Editors), New York : MacMillan Company, 1963, p. 417.
8.
Krawczyk, W.S.: A Pattern of Epidermal Cell Migration During Wound Healing, J Cell Biol49 :247-263, 1971.
9.
Pollard, T.D. and Weihing, R.R.: Actin and Myosin in Cell Movement, Crit Rev Biochem2:1-65, 1974.
10.
Goteiner, D. ; Krawczyk, W.S.; and Gillon, D.G.: Immunofluorescence of Anti-actin Antibody in Gingival Epithelium, J Perio Res12:436-443, 1977.
11.
Gabbiani, G. ; Chaponnier, C.; and Huttner, I.: Cytoplasmic Filaments and Gap Junctions in Epithelial Cells and Myofibroblasts During Wound Healing, J Cell Biol76:561-568, 1978.
12.
Pollard, T.D. and Korn, E.D.: Acanthamoeba Myosin, J Biol Chem248 :4682-4690, 1973.
13.
Pollard, T.D. ; Thomas, S.M.; and Niederman , R.: Human Platelet Myosin , Anal Bioch60:258-266, 1974.
14.
Clark, M. and Spudich, J.A.: Nonmuscle Contractile Proteins: The Role of Actin and Myosin in Cell Motility and Shape Determination, Annu Rev Bioch46:797-822, 1977.
15.
Niederman, R. and Pollard, T.D.: Human Platelet Myosin: II. In Vitro Assembly and Structure of Myosin Filaments, J Cell Biol67:72-92, 1975.
16.
Krawczyk, W.S. and Gillon, D.G.: Immunofluorescent Detection of Actin in Non-muscle Cells of the Developing Mouse Palatal Shelf, Arch Oral Biol21:503-508, 1976.
17.
Krawczyk, W.S. : Heavy Meromyosin Binding Microfilaments in Epidermal Cells During Wound Healing, Arch Derm Res258:63-68, 1977.