The biochemical function, adenylate energy charge (AEC), correlates with the viable count of S. mutans. AEC may be used to estimate the percent viable fraction of bacteria in dental plaque samples. An interactive computer program designed to process the AEC data is described.
Get full access to this article
View all access options for this article.
References
1.
Gibbons, R.J.; Socransky, S.S.; Araujo, W.C.; and Van Houte, J.: Studies of the Predominant Cultivable Microbiota of Dental Plaque. Arch. Oral Biol.9:365-370,1964.
2.
Loesche, W.J.; Hockett, R.N.; and Syed, S.A.: Predominant Cultivable Flora of Tooth Surface Plaque Removed from Institutionalized Subjects. Arch. Oral Biol.17:1311-1325, 1972.
3.
Bowden, G.H.; Hardie, J.M.; and Slack, G.L.: Microbial Variations in Approximal Dental Plaque. Caries Res.9:253-277,1975.
4.
Gordon, N.F.; Stutman, M.; and Loesche, W.J.: Improved Isolation of Anaerobic Bacteria from the Gingival Crevice Area of Man. Appl. Microbiol.21 :1046-1050, 1971.
5.
Robrish, S.A. ; Kemp, C.W.; and Bowen, W.H.: Use of Extractable Adenosine Triphosphate to Estimate the Viable Cell Mass in Dental Plaque Samples Obtained from Monkeys . Appl. Environ. Microbiol.35: 743-749, 1978.
6.
Forsberg, C.W. , and Lam, K.: Use of Adenosine 5'-triphosphate as an Indicator of the Microbiota Biomass in Rumen Contents. Appl. Environ. Microbiol. 33 :528-537,1977.
7.
Lee, C.C. ; Harris, R.F.; Williams, J.D.H.; Armstrong, D.E.; and Syers, J.K.: Adenosine Triphosphate in Lake Sediments . I. Determinations. Soil Sci. Proc.35:82-86, 1971.
8.
Lee, C.C. ; Harris, R.F.; Williams, J.D.H.; Syers, J.K.; and Armstrong , D.E.: Adenosine Triphosphate in Lake Sediments. II. Origin and Significance. Soil Sci. Proc.35:86-91,1971.
9.
Levin, G.V.; Chen, C.S.; and Davis, G.: Development of the Firefly Bioluminescent Assay for the Rapid Quantitative Detection of Microbial Contamination in Water. Aerospace Medical Research Laboratory Document no. TR6771. Defense Documentation Center , Alexandria, Va., 1967.
10.
Patterson, J.W.; Brezonik, P.L.; and Putnam, H.D.: Measurement and Significance of Adenosine Triphosphate in Activated Sludge. Environ. Sci. Technol.4:569-575,1970.
11.
Forrest, W.W. : Adenosine Triphosphate Pool During the Growth Cycle in Streptococcus faecalis. J. Bacteriol. 90:1013-1018, 1965.
12.
Atkinson, D.E. : The Energy Charge of the Adenylate Pool as a Regulatory Parameter. Interaction with Feedback Modifiers. Biochemistry7:4030-4034, 1968.
13.
Chapman, A.G. ; Fall, A.; and Atkinson, D.E.: Adenylate Energy Charge in Escherichia coli During Growth and Starvation. J. of Bacteriol.108:1072-1086, 1971.
14.
Montague, M.D. , and Dawes, E.A.: The Survival of Peptococcus prevotii in Relation to the Adenylate Energy Charge. J. of Gen. Microbiol . 80:291-299, 1974.
15.
Walker-Simmons, M. and Atkinson, D.E.: Functional Capacities and the Adenylate Energy Charge in Escherichia coli Under Conditions of Nutritional Stress. J. of Bacteriol. 130:676-683, 1977.
16.
Beny, M., and Dolivo, M.: Separation of Firefly Luciferase Using an Anion Exchanger. FEBS Letters70:167-170, 1976.
17.
Adam, H.: Adenosine-5'-diphosphate and Adenosine-5'-monophosphate, in Bergmeyer, H. V. (ed): Methods of Enzymatic Analysis, New York: Academic Press, Inc., 1963, pp 573-580.