By the use of the peroxidase-labeled antibody method, significant localization of IgG, IgA, albumin and transferrin was demonstrated in the deep lesion of 20 carious teeth, where the secretory component was absent. These serum proteins formed a distinct zone, surrounding the overlying, shallow lesion infected with bacteria.
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References
1.
Sumitani, M. ; Takeuchi, H.; Shima-Hara, T.; Tsubakimoto, K.; and Tsutsui, M.: Salivary, Serum and Microbial Components of Human Carious Dentin, J Dent Res51:1067-1070, 1972.
2.
Sainte-Marie, G.: A Paraffin Embedding Technique for Studies Employing Immunofluorescence, J Histochem Cytochem10: 250-256, 1962.
3.
Grabar, P.; and Williams, C.A.: Methode Permettant 1'E'tude Conjuguée des Propriétés E'lectrophorétiques et Immunochimiques d'un M61ange de Protéines. Application au Serum Sanguin; Biochim Biophys Acta10 :193-194, 1953.
4.
Ouchterlony, 0.: Handbook of Immunodiffusion and Immunoelectrophoresis, MichiganAnn Arbor Science Publishers, Inc., 1968, pp 21-47.
5.
Jones, I.L.; and Leaver, A.G.: Studies on the Minor Components of the Organic Matrix of Human Dentin, Archs Oral Biol19: 371-380,1974.
6.
Becker, W.: Determination of Antisera Titers Using the Single Radial Immunodiffusion Method , Immunochem6:539-546, 1969.
7.
Vaerman, J.-P. ; Heremans, J.F.; and Vaerman, C.: Studies of the Immune Globulins of Human Serum - LA. Method for the Simultaneous Isolation of the Three Immune Globulins (γss γ1M and γ1A) from Individual Small Serum Samples, J Immunol91:7-10, 1963.
8.
Nakane, P.K. ; and Kawaoi, A.: Peroxidase Labeled Antibody. A New Method of Conjugation, J Histochem Cytochem22: 1084-1091, 1974.
9.
Graham, R.C. ; and Karnovsky, M.J.: The Early Stages of Adsorption of Injected Horseradish Peroxidase in the Proximal Tubules of Mouse Kidney, Ultrastructural Cytochemistry by a New Technique, J Histochem Cytochem14:291-302,1966.
10.
Mancini, G. ; Carbonara, A.O.; and Heremans , J.F.: Immunochemical Quantitation of Antigens by Single Radial Immunodiffusion, Immunochem2:235-254, 1965.
11.
Brandtzaeg, P.; Fjellanger, I.; and Gjeruldsen , S.T.: Adsorption of Immunoglobulin A onto Oral Bacteria in vivo, J Bacteriol96:242-249, 1968.
12.
Nisengard, R.; and Jarrett, C.: Coating of Subgingival Bacteria with Immunoglobulin and Complement, J Periodontol47:518-521, 1976.
13.
Thomas, M.; and Leaver, A.G.: Identification and Estimation of Plasma Proteins in Human Dentin, Archs Oral Biol20:217-218, 1975.
14.
Steinman, R.R. ; and Hardinge, M.G.: The Effect of Pyridoxine and Injected Carboyhydrate on Incidence of Caries. Dentinal Circulation Related to Diet, J Dent Res37:874-879,1958.
15.
Steinman, R.R. : Pharmacologic Control of Dentinal Fluid Movement and Dental Caries in Rats, J Dent Res47:720-724, 1968.
16.
Steinman, R.R. ; and Leonora, J.: Relationship of Fluid Transport through the Dentin to the Incidence of Dentinal Caries, J Dent Res50:1536-1543, 1971.
17.
Steinman, R.R. ; and Leonora, J.: Effect of Infusing Selected Chemical Compounds on Dentinal Fluid Movement in the Rat, J Dent Res54:567-569, 1975.
