Enamel subsurface demineralization induced by Streptococcus mutans was significantly reduced by seven-day saliva pre-treatments conducive to the formation of enamel pellicles. A two-hour saliva pre-treatment was ineffective. Results suggest that the protection provided by long-term pellicles may relate to changes in ionic transport rates rather than cell attachment.
Get full access to this article
View all access options for this article.
References
1.
Dawes, C.; Jenkins, G.N.; and Tonge, C.H.: The nomenclature of the integuments of the enamel surface of teeth. Br. Dent. J. 115 :65-68,1963.
2.
Meckel, A.H.: The formation and properties of organic films of teeth. Arch. Oral Biol. 10:585-597, 1965.
3.
Mayhall, C.W.: Concerning the composition and source of the acquired enamel pellicle of human teeth. Arch. Oral Biol. 15 :1327-1341, 1970.
4.
Sonju, T.; and Rolla, G.: Chemical analysis of the acquired pellicle formed in two hours on cleaned human teeth in vivo. Caries Res. 7:30-38, 1973.
5.
Hay, D.I.: The adsorption of salivary proteins by hydroxyapatite and enamel. Arch. Oral Biol. 12:937-946, 1967.
6.
Saxton, C.A. : Scanning electron microscope study of the formation of dental plaque. Caries Res. 7:102-119, 1973.
7.
Mcgaughey, C. ; Field, B.D.; and Stowell, E.C.: Effects of salivary proteins on the adsorption of cariogenic Streptococci by hydroxyapatite. J. Dent. Res . 50:917-922, 1971.
8.
Rstavik, D.; Kraus, F.W.; and Hen-Shaw, L.C.: In vitro attachment of Streptococci to the tooth surface.Infect. Immun.9:794-800, 1974.
9.
Gibbons, R.J. ; Moreno, E.C.; and Spinell, D.M.: Model delineating the effects of a salivary pellicle on the adsorption of Streptococcus miteor onto hydroxyapatite. Infect. Immun. 14 :1109-1112,1976.
10.
Liljemark, W.F.; and Schauer, S.V.: Competitive binding among oral Streptococci to hydroxyapatite. J. Dent. Res. 56:157-165, 1977.
11.
Zahradnik, R.T.; Moreno, E.C.; and Burke, E.J.: Effect of saliva pellicle on enamel subsurface demineralization in vitro. J. Dent. Res. 55:664-670, 1976.
12.
Leach, S.A. ; and Saxton, C.A.: An electron microscope study of the acquired pellicle and plaque formed on the enamel of human incisors. Arch. Oral Biol. 11 :1081-1094, 1966 .
13.
Frank, R.M.; and Houver, G.: An ultrastructural study of human supragingival dental plaque formation; in McHugh, Dental Plaque (Thomas, Dundee) 1970.
14.
Newman, H.N. : The organic films on enamel surfaces. 1. The vestigial enamel organ. Brit. Dent. J. 135:64-67, 1973.
15.
Newman, H.N. : The organic films on enamel surfaces. 2. The dental plaque. Brit. Dent. J. 135: 101-106, 1973.
16.
Zahradnik, R.T.; Propas, D.; and Moreno, E.C.: In vitro enamel demineralization by Streptococcus mutans in the presence of salivary pellicles. J. Dent. Res. 56:1107-1110, 1977.
17.
Moreno, E.C.: The effect of acquired pellicle on enamel demineralization. Proc. Int. Symp. Acid Etch Tech, Silverstone, L. N. and Dogon, 1. L. (eds.): St. Paul, North Central Publ. Co., pp. 1-12, 1975.
18.
Krasse, B.: Human Streptococci and experimental caries in hamsters. Arch. Oral Biol. 11:429-436,1966.
19.
Donkersloot, J.A.; Robrish, S.A.; and Krichevsky , M.I.: Fluorometric determination of deoxyribonucleic acid in bacteria with ethidium bromide. Applied Microbiology24:179-183, 1972.
20.
Sonju, T.; Christensen, T.B.; Korn-Stad, L.; and Rolla, G.: Electron microscopy, carbohydrate analyses and biological activities of the proteins adsorbed in two hours to tooth surfaces in vivo. Caries Res. 8:113-122, 1974.
21.
Orstavik, D. ; and Kraus, F.W.: The acquired pellicle: Immunofluorescent demonstration of specific proteins. J. Oral Path. 2:68-76, 1973.
22.
Kraus, F.W. ; Örstavik, D.; Hurst, D.C.; and Cook, C.H.: The acquired pellicle: variability and subject-dependence of specific proteins. J. Oral Pathol.2 :165-173,1973.
23.
Belcourt, A. ; Frank, R.M.; and Houver, G.: Analyse des acids amines de la pellicule exogene acquise et des proteins de l'email superficiel chez l'homme. J. Biol. Buccale2:161-179, 1974.
24.
Lie, T. : Pellicle formation on hydroxyapatite splints attached to the human dentition: Morphologic confirmation of the concept of adsorption. Arch. Oral Biol. 20:739-742, 1975.
25.
Tinanoff, N. ; Glick, P.L.; and Weber, D.F.: Ultrastructure of organic films on the enamel surface. Caries Res.10:19-32, 1976. 26. Lie, T.: Scanning and transmission electron microscope study of pellicle morphogenesis. Scand. J. Dent. Res.85:217-231, 1977. 27. Moreno, E.C.; and Zahradnik, R.T.: Chemistry of enamel subsurface demineralization in vitro. J. Dent. Res.53:226-235, 1974.
26.
Turner, E.P. : The integument of the enamel surface of the human tooth: II. The acquired enamel cuticle. Dent. Practitioner8:373-382, 1958.
