Restricted accessResearch articleFirst published online 1978-11
Cellular Regulation of the Metabolism of Androgens in Rat Oral Mucosa: I. Effect of Medroxyprogesterone Acetate (MPA) on the Soluble Supernatant (Cytosol) Activating Microsomal Δ4-3-Ketosteroid-5α-Reductase and 17β -Hydroxysteroid Oxidoreductase Enzyme Systems
Soluble supernatant (cytosol) of rat oral mucosa was found to contain a factor accelerating the activity of microsomal Δ4-3-ketosteroid-5α-reductase and 17β-hydroxysteroid oxidoreductase enzymes metabolizing testosterone. Systemic administration of medroxyprogesterone acetate further increased this cytosolic activity.
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References
1.
Vittek, J.; Altman, K.; Rappaport, S.C.; Gordon, G.G.; Hagedoorn, J.; and Southren, A.L.: Effect of progestins on androgen Δ4-3-ketosteroid-5α-A-ring reductase system in rat oral mucosa, J Dent Res57 :511-519, 1978.
2.
Altman, K.; Gordon, G.G.; Southren, A.L.; Vittek, J.; and Wilker, S.: Induction of hepatic testosterone A-ring reductase by medroxyprogesterone acetate, Endocrinology90:1252-1260, 1972 .
3.
Gordon, G.G. ; Altman, D.; Southren, A.L.; and Olivo, J.: Human hepatic testosterone A-ring reductase activity: Effect of medroxyprogesterone acetate, J Clin Endocr32:457-461, 1971.
4.
Tseng, L. and Gurpide, E.: Induction of human endometrial estradiol dehydrogenase by progestins, Endocrinology97:825-833, 1975.
5.
Betz, G.; Tsai, P.; and Olson, G.: Stimulatory effect of soluble supernatant on hydroxylase activity of rat testes microsomes, Gynec Investig8:23-24, 1977.
6.
Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; and Randall, R.J.: Protein measurement with the Folin-Phenol reagent, J Biol Chem193:265-275, 1951.
