Collagenase activity was studied in human leukocytes, gingival crevicular
fluid and bacterial plaque, with soluble radioactive collagen as substrate.
Inflamed gingiva liberated vertebrate type collagenase into the crevicular fluid
in active form. Healthy gingiva, in contrast, released collagenase in a latent
form that could be activated by trypsin or plaque. Plaque also stimulated
leukocytes to release collagenase, and activated the latent enzyme.
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