Effect of Isoprenaline on Salivary Gland Lipids of Rats Fed a Choline-Deficient Diet

Abstract

Male adult rats were fed choline deficient and a choline-supplemented diet for 3 weeks. Half the rats from each group were injected with isoprenaline (16 mg/kg body wt.) and the remainder with saline. After 2 and 8 hours, rats were sacrificed, their submandibular salivary glands (SMSG) were disected out, and extracted for lipids. The fatty acid composition of total lipids and some neutral and phospholipid fractions were determined. Isoprenaline administration resulted in a slightly higher level of oleic acid in SMSG of rats fed choline-deficient but not the control diet. There was an increase in the free fatty acid (FFA) concentrations of the gland after 2 hours of isoprenaline treatment in rats fed the control diet; no such increase was observed in the choline-deficient group. The fatty acid composition of the FFA fraction was also slightly changed as a result of isoprenaline treatment, but only in the choline-deficient group. The fatty acid composition of triglyceride (TG), phosphatidyl choline (PC), and phosphatidyl ethanolamine (PE) fractions was not changed.

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References

Barka, T. : Stimulation of DNA Synthesis by Isoproterenol in the Salivary Gland, Exp Cell Res 39: 355-364, 1965a.

Schneyer, C.A. , and Shackleford, J.M. : Accelerated Development of Salivary Glands of Early Postnatal Rats Following Isoproterenol, Proc Soc Exp Biol Med 112:320-324, 1963.

Chan, W.C. : Enlargement of the Rats Salivary Gland and Salivary Calculus Formation Induced with Isoprenaline, J Path Bact 88:563-574, 1964.

Barka, T. : Induced Cell Proliferation. The Effect of Isoproterenol, Exp Cell Res 37: 662-679, 1965b.

Barka, T. : Stimulation of RNA Synthesis in the Salivary Gland by Isoproterenol, Exp Cell Res 41: 573-579, 1966.

Barka, T. : Stimulation of Protein and Ribonucleic Acid Synthesis in Rat Submaxillary Gland by Isoproterenol, Lab Invest 28 :38-41, 1968.

Baserga, R. , and Heffler, S. : Stimulation of DNA Synthesis by Isoproterenol and Its Inhibition by Actinomycin D. Exp Cell Res 46:571-580, 1967.

Radley, J.M. : Changes in Ploidy in the Rat Submaxillary Gland Induced by Isoprenoline, Exp Cell Res 48:679-681, 1967.

Schneyer, C.A. ; Finley, W.H. ; and Finley, S.C. : Increased Chromosome Number of Rat Parotid Cells After Isoproterenol, Proc Soc Exp Biol Med 125: 722-728, 1967. 10. Malmud, D. , and Baserga, R. : Glycogen Concentration and DNA Synthesis in Isoproterenol-Stimulated Salivary Glands, Exp Cell Res 50:581-588, 1968.

10.

Selye, H. ; Veilleaux, R. ; and Cantin, M. : Excessive Stimulation of Salivary Gland Growth by Isoproterenol, Science 133 :44-45, 1961.

11.

Byrt, P. : Secretion and Synthesis of Amylase in the Rat Parotid Gland After Isoprenaline , Nature (Lond.) 212:1212-1215, 1966 .

12.

Galanti, N. ; and Baserga, R. : Glycolipid Synthesis in the Early Prereplicative Phase of Isoproterenol-Stimulated Salivary Glands of Mice, J Biol Chem 246:6814-6821, 1971. 14. Alam, S.Q. , and Alam, B.S. : Effect of Feeding a Choline-Deficient Diet on Submandibular Salivary Gland Lipids of Rats, Arch Oral Biol 20:531-535. 1975.

13.

Navia, J.M. ; Lopez, H. ; and Harris, R.S. : Purified Diet for Dental Caries Research with Rats, J Nutr 97:133-140, 1969.

14.

Strength, D.R. ; Schaefer, E.A. ; and Salmon, W.D. : The Relation of Vitamin B12 and Folacin to the Utilization of Choline and Its Precursors for Lipotropism and Renal Protection in Rats, J Nutr 45 :329-343, 1951.

15.

Best, C.H. ; Lucas, C.C. ; Patterson, J.M. ; and Rideout, J.H. : Some Effects of Vitamin B12 in Weanling Rats Consuming Hypolipotropic Diets , Can J Med Sci 31: 135-145, 1953.

16.

Folch, J. ; Lees, M. ; and Sloane-Stanley, G.H. : A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J Biol Chem 226:497-509, 1957.

17.

Metcalfe, L.D. ; Schmitz, A.A. ; and Pelka, J.R. : Rapid Preparation of Fatty Acids Esters from Lipids for Gas Chromatographic Analysis, Anal Chem 38:514-515, 1966.

18.

Rouser, G. ; Kritchevesky, G. ; and Yam-Amato, A. : Column Chromatographic and Associated Procedures for Separation and Determination of Phosphatides and Glycolipids, in Lipid Chromatographic Analysis , Marinftti, G.V. (ed), New York: Dekker, 1967, Vol 1, pp 118-120.

19.

Skipski, V.P. ; Peterson, R.F. ; and Bar-Clay , M. : Quantitative Analysis of Phospholipids by Thin Layer Chromatography, Biochem J 90:374-378, 1964.

20.

Bartlett, G.R. : Phosphorus Assay in Column Chromatography, J Biol Chem 234: 446-468, 1959.

21.

Christi, W.C. : Hydrogenation, in Lipid Analysis, Oxford: Pergamon Press, 1973, pp 135.

22.

Hegsted, D.M. ; Roach, A.M. ; and Mc-Combs , H.L. : Effect of Vitamin B12 in Choline Deficiency in the Rat, J Nutr 92 :403-411, 1967.

23.

Newberne, P.M. ; Bresnahan, M.R. ; and Kula, N. : Effects of Two Synthetic Antioxidants, Vitamin E, and Ascorbic Acid on the Choline-Deficient Rat. J Nutr 97 :219-231, 1969.

24.

Beare-Rogers, J.L. : Liver Phospholipids of Rats Deprived of Dietary Choline. Can J Biochem 47:257-263, 1969.

25.

Burns, T.W. ; Langley, P.E. ; and Robin-Son , G.A. : Adrenergic Receptors and Cyclic AMP in the Regulation of Human Tissue Lipolysis, Ann NY Acad Sci 185:115-129, 1971.

26.

Himms-Hagen, J. : Sympathetic Regulation of Metabolism, Pharmacol Rev 19:367-461, 1967.

27.

Pritchard, E.T. : Investigation of Lipids and Lipid Metabolism in Submandibular Salivary Gland of the Rat, Arch Oral Biol 12:1443-1456, 1967.

28.

Griffith, W.H. , and Nyg, J.F. : Effects of Choline Deficiency, in The Vitamins, Seb-Rell, W.H. , and Harris, R.S. (eds), New York: Academic Press, 1971, Vol. III, pp 88-89.

29.

Alam, S.Q. , and Alam, B.S. : Effects of Essential Fatty Acid Deficiency on Salivary Glands, Submitted to J Nutr.

30.

Oron, Y. ; Lowe, M. ; and Selinger, Z. : Involvement of the a-Adrenergic Receptor in the Phospholipid Effect in Rat Parotid, FEBS Lett 34:198-200, 1973.