All seven oral Actinomycetaceae tested grew in broth containing 10 μg/ml Cd. After 24 hours' growth A viscosus WVU 626 bound nearly 20 μg Cd/mg dry wt. The amount of Cd bound varied during the growth cycle, being maximal (90 to 100 μg/mg dry wt.) after 5 hours' growth. Addition of 50 μg/ml chloramphenicol to actively growing cells inhibited further Cd binding. In contrast S mutans FA1 was greatly inhibited by 2.5 μg/ml Cd and bound only 1.3 μg Cd/mg after 24 hours' growth in broth containing 2.5 μg/ml Cd.
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