Human saliva was tested for the presence of factors that affect the complement system. Parotid saliva and salivary fractions were incubated at 37 C with human serum as a source of complement. Samples removed from the mixtures within the first 15 minutes had higher levels of whole hemolytic complement activity than did appropriate controls. The final ionic strength of the saliva-serum mixtures was critical to the hemolytic activity of complement. After 60 minutes all serum-saliva mixtures had lower levels of hemolytic activity than did serum-buffer controls. With regard to whole saliva, the salivary sediment was found to be strongly complement-reactive.
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