The antibacterial effect of purified human salivary lactoperoxidase on a cariogenic strain of Streptococcus mutans was demonstrated while another oral peroxidase, probably of leukocytic origin, did not affect the growth. Lactoperoxidase was rapidly adsorbed by bacterial cells indicating the necessity of the contact between the enzyme and the cells before inhibition.
Get full access to this article
View all access options for this article.
References
1.
Wright, R.C. , and Tramer, J.: Factors Influencing the Activity of Cheese Starters. The Role of Milk Peroxidase, J Dairy Res25:104-118, 1958.
2.
Jago, G.R., and Morrison, M.: Anti-Streptococcal Activity of Lactoperoxidase III, Proc Soc Exp Biol Med111:585-588, 1962.
3.
Reiter. B.; Pickering, A.; and Oram, J.D.: in Microbial Inhibitors in Food, by Molin, N., (ed), Stockholm, Almqvist and Wiksell, 1964, p 297.
4.
Klebanoff, S.J., and Luebke, R.G.: The Antilactobacillus System of Saliva. Role of Salivary Peroxidase , Proc Soc Exp Biol Med118:483-486, 1965.
5.
Klebanoff, S.J. ; Clem, W.H.; and Luebke, R.G.: The Peroxidase-Thiocyanate·Hydrogen Peroxide Antimicrobial System , Biochim Biophys Acta117:63-72, 1966.
6.
Oram, J.D., and Reiter, B.: The Inhibition of Streptococci by Lactoperoxidase, Thiocyanate and Hydrogen Peroxide. The Effect of the Inhibitory System on Susceptible and Resistant Strains of Group N Streptococci, J Biochem100:373-381, 1966.
7.
Dogon, I.L. ; Kerr, A.C.; and Amdur, B.H.: Characterization of an Antibacterial Factor in Human Parotid Secretions, Active Against Lactobacillus casei, Arch Oral Biol7:81-90, 1962.
8.
Zeldow, B.J. : Studies on the Antibacterial Action of Human Saliva. III. Cofactor Requirements of a Lactobacillus Bactericidin, J Immunol90:12-16, 1963.
9.
Iwamoto, Y., and Matsumura, T.: Purification and Characterization of the Salivary Antibacterial Factor (S.A. Factor), Arch Oral Biol11:667-676, 1966.
10.
Klebanoff, S.J. ; Luebke, R.G.; and Clem, W.H.: Studies on an Antibacterial System of Saliva, IADR Abstracts No 199, p 86, 1965.
11.
Hoogendoorn, H., and Moorer, W.R.: Lactoperoxidase in the Prevention of Plaque Accumulation, Gingivitis and Dental Caries (I), Odontol Revy24:355-366. 1973.
12.
Morrison, M. ; Allen, P.Z.; Bright , J.; and Jayasinghe , W.: Lactoperoxidase. V. Identification and Isolation of Lactoperoxidase from Salivary Gland, Arch Biochem Biophys111:126-133, 1965.
13.
Morrison, M., and Steele, W.F.: Lactoperoxidase, the Peroxidase in the Salivary Gland, in Biology of the Mouth, Person, P., (ed), Washington, 1968, pp 89-110.
14.
Mäkinen, K.K. , and Tenovuo, J.: Chromatographic Separation of Human Salivary Peroxidases, Acta Odont Scand34:141-150, 1976.
15.
Chance, B., and Maehly, A.C.: in Methods in Enzymology, Vol II. Academic Press , New York, 1964, p 764.
16.
Makinen, K.K. ; Tenovuo, J.; and Scheinin, A.: Xylitol-Induced Elevation of Lactoperoxidase Activity, J Dent Res55:652-660, 1976.
17.
Lowry, O.H. ; Rosebrough, N.J. ; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193:265-275,1951.
18.
Powell, W.N. : Photoelectric Determination of Blood Thiocyanates without Precipitation of Proteins, J Lab Clin Med30:1071-1075, 1945.
19.
Knuuttila, M.L.E., and Mäkinen , K.K.: Purification and Characterization of a Phosphatase Specifically Hydrolyzing p-Nitrophenyl Phosphate from an Oral Strain of Streptococcus mutans, Arch Biochem Biophys152:685-701, 1972.
20.
Hoogendoorn, H.: The Effect of Lactoperoxidase-Thiocyanate-Hydrogen Peroxide on the Metabolism of Cariogenic Microorganisms In Vitro and In the Oral Cavity, Academic dissertation, Haag, Holland, 1974.
21.
Tenovuo, J. , and Valtakoski, J.: The Correlation Between Salivary Peroxidase Activity, Salivary Flow Rate, and the Oxidation-Reduction Potentials of Human Saliva and Dental Plaque Suspensions, Acta Odont Scand34:169-176,1976.
22.
Iwamoto, Y. ; Nakamura, R.; Tsunemitsu , A.; and Matsumura, T.: The Heterogeneity of Human Salivary Peroxidase , Arch Oral Biol13:1015-1018, 1968 .
23.
Slowey, R.R. ; Eidelman, S.; and Klebanoff, S.J.: Antibacterial Activity of the Purified Peroxidase from Human Parotid Saliva, J Bacteriol96:575-579,1968.
24.
Hogg, D.McC. , and Jago, G.R.: The Antibacterial Action of Lactoperoxidase. The Nature of the Bacterial Inhibitor, Biochem J117:779-790,1970.