The Seeded Growth of Calcium Phosphates on Dentin and Predentin

Abstract

The in vitro mineralization of predentin (PD) and of the organic component of dentin (OD) has been studied in a supersaturated solution of calcium phosphate at 37 C and pH = 7.4 at total calcium and phosphate concentrations close to those in vivo. The mineralization of OD can be divided into at least three periods based upon the morphology and the specific surface area of the mineral phase formed. Dissolution of the newly precipitated mineral on OD revealed a stoichiometry close to that for octacalcium phosphate. In contrast, PD was ineffective as a crystal nucleator. The mineralization of the inorganic component of dentin (ID) and whole dentin (D) has also been examined.

Get full access to this article

View all access options for this article.

References

Meyer, J.L. , and Nancollas, G.H. : The Effect of Stannous and Fluoride Ions on the Rate of Crystal Growth of Hydroxyapatite, J Dent Res 51:1443-1449, 1972.

Meyer, J.L. , and Nancollas, G.H. : The Effect of pH and Temperature on the Crystal Growth of Hydroxyapatite , Arch Oral Biol 17:1623-1627, 1972 .

Nancollas, G.H. , and Mohan, M.S. : The Growth of Hydroxyapatite Crystals, Arch Oral Biol 15:731-745, 1970.

Nancollas, G.H. : Physicochemistry of Demineralization and Remineralization , J Dent Res 53: 297-302, 1974.

Glasstone, S. : Experimental Studies on Calcification of Tooth Germs In Vitro, J Dent Res 37:738-747, 1958.

Dietz, V.R. , and Gee, A. : Determination of Phosphate by Differential Spectrophotometry, Anal Chem 25:1320-1324, 1953 .

Culling, V.F.A. : Handbook of Histopathological Techniques, London , England: Butterworths, 1973.

Swedlow, D.B. ; Harper, R.A. ; and Katz, J.L. : Evaluation of a New Preparative Technique for Bone Examination in the SEM, from Scanning Electron Microscopy/1972 (Part II), Proceedings of the Workshop on Biological Specimen Preparation Techniques for Scanning Electron Microscopy, Chicago, Illinois, IIT Research Institute, 1972.

Sarnat, B. , and Massler, M. : Microstructure of Active and Arrested Dentinal Caries, J Dent Res 44:1389-1401, 1965.

10.

Tomazic, B. , and Nancollas, G.H. : The Seeded Growth of Calcium Phosphates , J Colloid Interfacial Sci 50:451, 1975.