Human fibroblasts have been cultured in the presence of Diphenylhydantoin. No consistent stimulation of cell growth was observed. The drug at a concentration of 0.02 mM, in the presence of ascorbic acid, decreased collagen prolyl hydroxylation in "old" W1-38 cultures but a concentration of 0.1 mM was required to decrease prolyl hydroxylation in "young" cultures.
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References
1.
Merritt, H.H. , and Putnam, T.J.: Sodium Hydantoinate in the Treatment of Convulsive Disorders, JAMA111:1068-1073, 1938.
Harbison, R.D. , and Becker, B.A.: Relation of Dosage and Time of Administration of Diphenylhydantoin to Its Teratogenic Effect in Mice, Teratology2:305-312, 1969.
6.
Harbison, R.D. , and Becker, B.A.: Comparative Embryotoxicity of Diphenylhydantoin and Some of Its Metabolites in Mice, Teratology10:237-242, 1974.
7.
Miller, R.P. , and Becker, B.A.: Teratogenicity of Oral Diazepam and Diphenylhydantoin in Mice, Toxicol Appl Pharmacol32:53-61, 1975.
8.
Poswillo, D.E. : The Pathogenesis of Sub-mucous Cleft Palate , Scand J Plast Reconstr Surg8:34-41, 1974.
9.
Shafer, W.G. : Effect of Dilantin Sodium on Growth of Human Fibroblast-like Cell Cultures, Proc Soc Exp Biol Med104 :198-201, 1960.
10.
Shafer, W.G. : Effect of Dilantin Sodium Analogues on Cell Proliferation in Tissue Culture, Proc Soc Exp Biol Med106 :205-207, 1961.
11.
Shafer, W.G. : Effect of Dilantin Sodium on Various Cell Lines in Tissue Culture, Proc Soc Exp Biol Med108:694-696, 1961.
12.
Shafer, W.G. : Response of Radiated Human Gingival Fibroblast-like Cells to Dilantin Sodium in Tissue Cultures, J Dent Res44:671-677, 1965.
13.
Nease, W.J. : Effect of Sodium Diphenyl-hydantoinate on Tissue Cultures of Human Gingiva, J Periodontol36:22-33, 1965.
14.
Noess, T.: The Effect of 5,5-Diphenylhydantoin on Fibroblast-like Cells in Culture , J Periodont Res4:163-164, 1969.
15.
Robinfaux, R. ; Lorans, G. ; and D'Augeres , C.B.: Action of Diphenylhydantoin on the Growth and Respiration of Cells in Culture, Rev Europ Etudes Clin et Biol15: 1066-1971, 1970 .
16.
Yoshizumi, T.: Experimental Study of Tissue Culture Relating to the Etiology of 5,5-Diphenylhydantoin Gingival Hyperplasia, Shikwa Gakuho71:183-207, 1971.
17.
Cristofalo, V.J., and Sharf, B.B.: Cellular Senescence and DNA Synthesis, Exp Cell Res76:419-427, 1973.
18.
Paz, M.A. , and Gallop, P.M.: Collagen Synthesized and Modified by Aging Fibroblasts in Culture, In Vitro11:302-312, 1975.
19.
Houck, J.C. ; Cheng, R.F.; and Waters, M.D.: The Effect of Dilantin upon Fibroblast Proliferation, Proc Soc Exp Biol Med139:969-971, 1972.
Gallop, P.M. , and Paz, M.A.: Posttranslational Protein Modifications, with Special Attention to Collagen and Elastin, Physiological Reviews55:418-487, 1975.
23.
Blumenkrantz, N., and Asboe-Hansen , G.: Connective-Tissue Effects of Drugs Causing Lupoid Syndrome, Acta Derm54 :35-38, 1974.
24.
Blumenkrantz, N., and Asboe-Hansen , G.: Effect of Diphenylhydantoin on Connective Tissue, Acta Neurol Scand50 :302-306, 1974.
25.
Chen, K.; Paz, M.A.; and Gallop, P.M.: Collagen Prolyl Hydroxylation in W 1-38 Fibroblast Cultures: Actions of Hydralazine , In Vitro13:49-54, 1977.
26.
Liu, T.Z. , and Bhatnagar, R.S.: Inhibition of Protocollagen Proline Hydroxylase by Dilantin, Proc Soc Exp Biol Med142 :253-255, 1973.
27.
Gallop, P.M. ,; Blumenfeld, O.O.; and Seifter, S.: Structure and Metabolism of Connective Tissue Proteins, Ann Rev Biochem41:617-671, 1972.
28.
Steffek, A.J. ; Verrusio, A.C.; and Wat-Kins , C.A.: Cleft Palate in Rodents after Maternal Treatment with Various Lathyrogenic Agents, Teratology5:35-40, 1972.
