Eighteen different Streptococcus strains including S mutans, sanguis, mitis, salivarius, bovis, and 2F2, were assayed for their relative adsorption to synthetic hydroxyapatite and for quantities of lactic acid produced while in the adsorbed state. The results indicated that all S mutans and some S sanguis have an enhanced metabolic activity while adsorbed to hydroxapatite as compared to control suspensions without hydroxyapatite.
Get full access to this article
View all access options for this article.
References
1.
Orland, F.J. ; Blayney, J.R.; Harrison , R.W.; Reyniers, J.A.; Trexler, P.C.; Erwin, R.E.; Gordon, H.A.; and Wagner, M.: Experimental Caries in Germ Free Rats Inoculated with Enterococci, JADA50: 259-272, 1955.
2.
Gibbons, R.J. , and Fitzgerald, R.J.: Dextran-Induced Agglutination of Streptococcus mutans, and Its Potential Role in the Formation of Microbial Dental Plaques , J Bact98:341-346, 1969.
3.
Mcgaughey, C. ; Field, B.D.; and Stow-Ell, E.C.: Effects of Salivary Proteins on the Adsorption of Cariogenic Streptococci by Hydroxyapatite, J Dent Res50:917-922, 1971.
4.
Zoeell, C.E.: The Effect of Solid Surfaces Upon Bacterial Activity, J Bact46:39-55, 1943.
5.
Hendricks, C.W.: Sorption of Heterotropic and Enteric Bacteria to Glass Surfaces in the Continuous Culture of River Water, Applied Micro28:572-578, 1974.
6.
Stotzky, G., and Rem, L.T.: Influence of Clay Minerals on Microorganisms. I. Montmorillonite and Kaolinite on Bacteria, Can J Micro12:547-563, 1966.
7.
Lahav, N., and Keynan, A.: The Influence of Bentonite and Attapulgite on the Respiration of Bacillus subtilis, Can J Micro8:565-572, 1962.
8.
Hattori, T., and Furusaka, C.: Chemical Activities of Escherichia coli Adsorbed on a Resin, J Biochem48:831-837, 1960.
9.
Tschapek, M. , and Giambiage, N. : Bacteria Metabolism in Adsorbed State, Trans 6 Congr Soil Sci 8C3: 181-187, 1956.
10.
Giesons, R.J. , and Banghart, S.: Induction of Dental Caries in Gnotobiotic Rats with a Levan Forming Streptococcus and a Streptococcus Isolated From Subacute Bacterial Endocarditis, Arch Oral Biol13 :297-308, 1968.
11.
Gillings, B.R.D. , and Beck, D.J. : Tooth Enamel Powders for Experimental Procedures, J Dent Res43: 304, 1964.
12.
Good, N.E.; Winget, G.D.; Winter, W.; Connally, T.N.; Izawa, S.; and Singh, R.M.M.: Hydrogen Ion Buffers for Biological Research, Biochem5:467-477, 1966.
13.
Eagle, H.: Buffer Combinations for Mammalian Cell Culture, Science174:500-503. 1971.
14.
Berry, C.W., and Henry, C.A.: Studies of Streptococcal Cell Adsorption Using a Membrane Filter Technique , IADR Program and Abstract of Papers No. 285, 1973.
15.
Berry, C.W., and Henry, C.A.: The Influence of Adsorption on the Metabolism of Streptococcus mutans and sanguis, IADR Program and Abstract of Papers No. 229. 1975 .
16.
Barker, S.B. , and Summerson, W.H.: The Colorimetric Determination of Lactic Acid in Biological Materials, J Biol Chem138:535-554, 1941.
17.
Bonting, S.L. : The Colorimetric Determination of Lactic Acid in Submicrogram Quantities, Arch Biochem Biophys56 :307-317, 1955.
18.
Hillman, J.D. ; Van Houte, J.; and Gibbons, R.J. : Sorption of Bacteria to Human Enamel Powder , Arch Oral Biol15 :899-903, 1972.
19.
Schachtele, M.S.: Recent Developments in the Microbiology of Dental Plaque Formation, Northwest Dentistry51:108-113. 1972.
20.
Cotton, W.R.; Moody, R.W.; and Lyon, H.W.: Streptococcus mutans Colonization and Effect on Enamel Surfaces, IADR Program and Abstract of Papers, No. 287, 1973.
21.
Van Houte, J.; Gibbons, R.J.; and Bang. Hart, S.B.: Adherence as a Determinant of the Presence of Streptococcus salivarius and Streptococcus sanguis on the Human Tooth Surface , Arch Oral Biol15:1025-1934, 1970 .
22.
Van Houte, J.; Gibbons, R.J.; and Pulk-Kinen , A.J.: Adherence as an Ecological Determinant for Streptococci in the Human Mouth, Arch Oral Biol16:1131-1141, 1971.
23.
Gibbons, R.J. , and Van Houte, J.: Selective Bacterial Adherence to Oral Epithelial Surfaces and Its Role as an Ecological Determinant, Infect and Immunity3:567-573, 1971.
24.
Bratthall, D.: Demonstration of Five Serological Groups of Streptococcal Strains Resembling Streptococcus mutans, Odontol Revy21 :143-152, 1970.
25.
Estermann, E.F., and McLaren, A.D.: Stimulation of Bacterial Proteolysis by Adsorbents, J Soil Sci10:64-78, 1959.
26.
Stotzky, G. : Influence of Clay Minerals on Microorganisms. II. Effect of Various Clay Species, Homoionic Clays, and Other Particles on Bacteria, Can J Microbiol12 :831-848, 1966.
27.
Stotzky, G. : Influence of Clay Minerals on Microorganisms. III. Effect of Particle Size, Cation Exchange Capacity, and Surface Area on Bacteria, Can J Microbiol12: 1235-1246, 1966.
