Phagocytosis by a human gingival epithelial-like cell line in tissue culture was studied using latex beads as a marker. Electron microscopic and chemical analyses revealed that the cells ingested the beads in a quantitative and reproducible manner. This phagocytosis system is potentially useful for studying the effects of dental plaque on cell metabolism.
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References
1.
Platt, H.: The Engulfment of Particulate and Collodial Materials by Epidermal Cells, J Path Bact86:113-222, 1963.
2.
Wolff, K., and Konrad, K.: Phagocytosis of Latex Beads by Epidermal Keratinocytes In Vivo, J Ultrastruct Res39:262-280, 1972.
3.
Maccallum, D.K. , and Scaletta, L.J.: Possible Phagocytosis by Keratinocytes: Ultrastructural Evidence from the Study of Human Oral Mucosa in Organ Culture , Arch Oral Biol18:449-455, 1973.
4.
Lange, D., and Schroeder, H.E.: Cytochemistry and Ultrastructure of Gingival Sulcus Cells, Helv Odont Acta15 (Sup. VI) :65-86, 1971.
5.
Page, R.C.; Simpson, M.D.; Ammons, W.F.; and Schectman, L.R.: Host Tissue Response in Chronic Periodontal Disease, J Periodont Res7:283-296, 1972.
6.
Mcmillam, M.D.: An Ultrastructural Study of the Relationship of Oral Bacteria to the Epithelium of Healing Tooth Extraction Wounds, Arch Oral Biol20:815-822, 1975.
7.
Takarada, H.; Cattoni, M.; Sugimoto, A.; and Rose, G.G.: Ultrastructural Studies of Human Gingiva. II. The Lower Part of the Pocket Epithelium in Chronic Periodontitis, J Perio45:155-169, 1974.
8.
Smulow, J.B., and Glickman, I.: An Epithelial-like Cell Line in Continuous Culture From Normal Adult Human Gingiva, Proc Soc Exp Biol Med. 121:1294-1296, 1966.
9.
Ulrich, F., and Zilversmit, D.B. : Release From Alveolar Macrophages of An Inhibitor of Phagocytosis, Am J Physio218 :1118-1127, 1970.
10.
Roberts, J. , and Quastel, L.: Particle Uptake by Polymorphonuclear Leucocytes and Erhlich Ascites-Carcinoma Cells, Biochem J89:150-156, 1963.
11.
Roth, T.F., and Porter, K.R.: Yolk Protein Uptake in the Oocyte of the Mosquito Aedes aegypti, J Cell Biol20: 313-332, 1964.
12.
Cobb, C., and Brown, L.R.: The Effects of Exudate From the Periodontal Pocket on Cell Culture , Periodont5:5-18, 1967.
13.
Baboolal, R. ; Milnek, A.; and Powell, R.N.: A Study of the Effects of Gingival Plaque Extracts on Cells Cultured In Vitro, J Periodont Res5:248-254, 1970.
14.
Levine, M.; Adams, R.L.P. ; Cowley, G.C.; and Mason, D.K.: Some Characteristics of the Cytotoxic Material in Human Dental Plaque Extracts , Arch Oral Biol19 :1145-1152, 1974 .
15.
Attström, R. : The Roles of Gingival Epithelium and Phagocytosing Leukocytes in Gingival Defense, J Clin Periodont2:25-32, 1975.
16.
Davis, B.D.; Dulbecco, R.; Eisen, H.N.; Ginsberg, H.S.; Wood, W.B.; and Mc-Carty, M.: Microbiology, 2nd ed., New York : Harper & Row, Publishers, Inc., p 650, 1973.
17.
Armstrong, J.A., and D'Arcy Hart , P.: Response of Cultured Macrophages to Mycobacterium tuberculosis With Observations on Fusion of Lysosomes with Phagosomes, J Exp Med134:713-740, 1971.
