Proteolipid was demonstrated to contain the nucleator of bone matrix calcification, in vitro. Crude Phospholipid extracted from bone matrix was fractionated by gel filtration. A single, protein-containing fraction induced apatite crystallization in a metastable calcium phosphate solution. The fraction was identified as proteolipid. The result supports the validity of a microbiologic analogue for vertebrate calcification.
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References
1.
Ennever, J., and Creamer, H.: Microbiologic Calcification: Bone Mineral and Bacteria, Calc Tiss Res1:87-93, 1967.
2.
Ennever, J.; Vogel, J.J.; and Takazoe, I.: Calcium Binding by a Lipid Extract of Bacterionema matruchotii, Calc Tiss Res2: 296-298, 1968.
3.
Takazoe, I.; Vogel, J.J.; and Ennever, J.: Calcium Hydroxyapatite Nucleation by Lipid Extract of Bacterionema matruchotii , J Dent Res49:395-398, 1970.
4.
Ennever, J.; Vogel, J.J.; and Levy, B.M.: Lipid and Bone Matrix Calcification In Vitro, Proc Soc Exp Biol Med, 145:1386-1388, 1974 .
5.
Ennever, J.; Vogel, J.J.; Rider, L.J.; and Boyan-Salyers, B. : Nucleation of Microbiologic Calcification by Proteolipid, Proc Soc Exp Biol Med152:147-150, 1976.
6.
Liu, T.Y.: Determination of Tryptophan, in Hirs, C.H.W. and Timasheff, S.N. (eds) : Methods in Enzymology, vol XXV. B, New York: Academic Press, 1972, pp 44-55.
7.
Rouser, G.; Kritchevsky, G.; and Yamamo-To, A.: Column Chromatographic and Associated Procedures for Separation and Determination of Phosphatides and Glycolipids, in Marinetti (ed) : Lipid Chromatographic Analysis, vol 1 , New York: Marcel Dekker Inc. , 1967 , pp 139-150.
8.
Bandurski, R.S. , and Axelrod, B.: The Chromatographic Identification of Some Biologically Important Phosphate Esters, J Biol Chem193:405-410, 1951.
9.
Folch, J., and Lees, M.: Proteoiipids, a New Type of Tissue Lipoproteins. Their Isolation from Brain , J Bio Chem191:807-817, 1951.
10.
Folch-Pi, J. , and Sakura, J.D.: Preparation of the Proteolipid Apoprotein from Bovine Heart, Liver and Kidney, Biochim Biophys Acta427:410-427, 1976.
11.
Folch-Pi, J. , and Stoffyn, P.J.: Proteolipids from Membrane Systems , Ann N Y Acad Sci195:86-107, 1972.
12.
Vogel, J.J. ; Campbell, M.M.; and Ennever, J.: Calcification of a Lyzozyme - Inositol Phosphatide Complex In Vitro, Proc Soc Exp Biol Med143:677-680, 1973.
13.
Dubreuil, G. : Vacuoles Lipoides des Osteoblastes, des Celluler Osseuses et des Osteoclastes, Comp Rend Soc Biol69:189-190, 1910.
14.
Irving, J.T. : Sudanophil Inclusions in Ameloblasts, Odontoblasts and Cells of the Oral Epithelium, Nature181:569-570, 1958.
15.
Irving, J.T. : A Histological Stain for Newly Calcified Tissue , Nature181:704-705, 1958.
16.
Wuthier, R.E. : Lipids of Mineralizing Epiphyseal Tissues in the Bovine Fetus, J Lipid Res9:68-78, 1968.
Peress, N.S. ; Anderson, H.C.; and Sajdera, S.W.: The Lipids of Matrix Vesicles from Bovine Fetal Epiphyseal Cartilage, Calc Tiss Res14:275-281, 1974.
19.
Shapiro, I.M. : The Lipids of Skeletal and Dental Tissues: Their Role in Mineralization, in Zipkin, 1. (ed) : Biological Mineralization, New York: Wiley Interscience, 1973 , pp 117-138.
