Influence of Growth Hormone and Thyroxine on Endochondral Osteogenesis in the Mandibular Condyle and Proximal Tibial Epiphysis

Abstract

Interrelationships of growth hormone and thyroxine effects on endochondral osteogenesis have been investigated. Evidence is offered that thyroxine augments the effect of growth hormone on the mandibular condyle just as at other osteogenetic centers, for example proximal tibial epiphysis in hypophysectomized rats. This contrasts with previous reports of a unique thyroxine antagonism to the effect of growth hormone on condylar development. In a further study on tibial epiphysis, special attention was given to assure absence of the endogenous thyroid hormone by adding thyroidectomy to hypophysectomy and reducing the possibility of thyroteopin residues in growth hormone. By histochemical tests, the responses of both calcified and non calcified regions of the epiphyseal cartilage plate were measured. Graded growth hormone doses stimulated graded response in a noncalcified region. Thyroxine augmented this response eightfold. The width of the calcified region of the plate did not vary with treatment.

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References

Enlow, D.H. : The Human Face: An Account of the Postnatal Growth and Development of the Craniofacial Skeleton, New York: Harper & Row, 1968, pp 52, 53, 110.

Shapiro, R. , and Janzen, A.H. : The Normal Skull (A Roentgen Study), New York: Paul B. Hoeber, Inc., 1960, pp 8-12.

Baume, L.-J. : Principles of Cephalofacial Development Revealed by Experimental Biology, Am J Orthod 47:881-901, 1961.

Baume, L.-J. : The Postnatal Growth Activity of the Nasal Cartilage Septum, Helv Odontol Acta 5:9-13, 1961.

Baume, L.-J. : Patterns of Cephalofacial Growth and Development: A Comparative Study of the Basicranial Growth Centers in Rat and Man, Int Dent J 18:489-513, 1968.

Asling, C.W. , and Evans, H.M. : Anterior Pituitary Regulation of Skeletal Development, in Bourne, G.H. (ed) : Biochemistry and Physiology of Bone, New York: Academic Press, Inc., 1956, pp 671-703.

Asling, C.W. ; Simpson, M.E. ; Li . C.H. ; and Evans, H.M. : The Effect of Chronic Administration of Thyroxine to Hypophysectomized Rats on Their Skeletal Growth, Maturation and Response to Growth Hormone, Anat Rec 119:101-117, 1954.

Urist, M.R. : Growth Hormone and Skeletal Tissue Metabolism, in Bourne, G H. (ed) : Biochemistry and Physiology of Bone, Vol 2, 2nd ed, New York : Academic Press, Inc., 1972, pp 155-195.

Kember, N.F. , and Walker, K.V.R. : Control of Bone Growth in Rats, Nature 229: 428-429, 1971.

10.

Thorngen, K.-C. , and Hansson, L.I. : Bioassay of Growth Hormone: I. Determination of Longitudinal Bone Growth with Tetracyline in Hypophysectomized Rats, Acta Endocrinol 75:653-668, 1974.

11.

Thorngren, G.-G. , and Hansson , L.I. : Conditions Influencing the Dose-Response Effect of Growth Hormone on Longitudinal Bone Growth in the Hypophysectomized Rat, Acta Endocrinol 80:284-296, 1975.

12.

Becks, H. ; Simpson, M.E. ; Evans, H.M. ; Ray, R.D. ; Li, C.H. ; and Asling, C.W. : Response to Pituitary Growth Hormone and Thyroxine of the Tibias of Hypophysecto-. mized Rats after Long Postoperative Intervals, Anat Rec 94:631-653, 1946.

13.

Collins, D.A. ; Becks, H. ; Simpson , M.E. ; and Evans, H.M. : Growth and Transformation of the Mandibular Joint in the Rat: I. Normal Female Rats; II. Hypophysectomized Female Rats; III. The Effect of Growth Hormone and Thyroxine Injections in Hypophysectomized Rats, Am J Orthod 32:431-451, 1946.

14.

Asling, C.W. ; Durbin, P.W. ; Parrott , M.W. ; Johnston, M.E. ; and Hamilton , J.G. : Evidence for Function of Aberrant Thyroid Tissue in Thymus of Rats, Proc Soc Exp Biol Med 94:200-211, 1957.

15.

Geschwind, I.I. , and Li, C.H. : The Tibia Test for Growth Hormone, in Smith, R.W. , Gaebler, O.H. , and Long, C.W.H. (eds): Hypophyseal Growth Hormone: Nature and Actions, New York: McGraw Hill, 1955, pp 28-51.

16.

Meier, S. , and Solursh, M. : The Comparative Effects of Several Mammalian Growth Hormones on Sulphate Incorporation into Acid Mucopolysaccharides by Cultured Chick Embryo Chondrocytes , Endocrinol 90:1447-1451, 1972 .

17.

Toole, B.P. : Hyaluronate Inhibition of Chondrogenesis: Antagonism of Thyroxine, Growth Hormone, and Calcitonin, Science 180:302-303, 1973.

18.

Geschwind, I.I. , and Li, C.H. : Reaction of Several Protein Hormones with Periodate, Endocrinol 63:449-459, 1958.

19.

Ingalls, T.H. : Epiphyseal Growth: Normal Sequence of Events at the Epiphyseal Plate, Endocrinol 29:710-719, 1941.

20.

Becks, H. ; Simpson, M.E. ; and Evans, H.M. : Ossification at the Proximal Tibial Epiphysis in the Rat I. Changes in Females with Increasing Age, Anat Rec 92:109-119, 1945.

21.

Becks, H. : SIMPSON, M.E.; and EVANS, H.M.: Ossification at the Proximal Tibial Epiphysis in the Rat I. Changes in Females at Progressively Longer Intervals Following Hypophysectomy, Anat Rec 92:121-133, 1945.

22.

Kibrick, E.A. ; Becks, H. ; Marx , W. ; and Evans, H.M. : The Effect of Different Dose Levels of Growth Hormone on the Tibia of Young Hypophysectomized Female Rats, Growth 5:437-447, 1941.

23.

Herbai, G. : Autoradiographic Studies with 35S-Sulfate on Somatotrophin and Estrogen Sensitive Growth Zones in Rat and Mouse Cartilage, Acta Physiol Scand 79:351-358, 1970.