Human gingival fibroblast cell lines were initiated in flask cultures from four periodontal patients with the diagnoses of periodontitis (two patients), fibromatosis, and Dilantin hyperplasia. The collagenolytic propensity of these fibroblasts cultivated on collagen-coated cover slips and the inhibitory effect of serum were evaluated by direct microscopic observations.
Get full access to this article
View all access options for this article.
References
1.
Beutner, E.H. ; Thriftshauser, C. ; and Hazen, S.P.: Collagenase Activity of Gingival Tissue from Patients with Periodontal Diseases, Proc Soc Exp Biol Med121 :1082-1085,1966.
2.
Fullmer, H.M. , and Gibson, W.: Collagenolytic Activity in Gingivae of Man , Nature209:728-729, 1966.
3.
Gibson, W., and Fuller, H.: Collagenolytic Activity of Gingival Tissues In Vitro, J Dent Res45:1225, 1966.
4.
Bennick, A., and Hunt, A.M.: Collagenolytic Activity in Oral Tissues, Arch Oral Biol12:1-9, 1967.
5.
Taylor, A.C. : Collagenolysis in Cultured Tissue: 1. Digestion of Mesenteric Fibers by Enzymes from Explanted Gingival Tissue, J Dent Res50:1294-1300, 1971.
6.
Rose, G.G., and Cattoni, M.: Human Gingiva Cultivated in Circumfusion Systems, Arch Oral Biol19:113-123, 1974.
7.
Fullmer, H.M. ; Gibson, W.A. ; Lazarus, G.S.; Bladen, H.A.; and Whedon, K.A.: The Origin of Collagenase in Periodontal Tissues of Man, J Dent Res48:646-651, 1969.
8.
Golub, L.M.; Kennett, S.; Micewan, H.; Curran, J.B.; and Ramamurthy, N.S. : Collagenolytic Activity of Crevicular Fluid from Pericoronal Gingival Flaps, J Dent Res55:157-181, 1976.
9.
Macdonald, J.B. ; Socransky, S.S.; and Gibbons, R.J.: Aspects of the Pathogenesis of Mixed Anerobic Infections of Mucous Membranes, J Dent Res42:529-544, 1963.
10.
Alfano, M.C. ; Morhart, R.E.; Metcalf , G.; and Drummond, J.F.: Presence of Collagenase from Clostridium histolyticum in Gingival Sulcal Debris of a Primitive Population, J Dent Res53:142, 1974.
11.
Lazarus, G.S. ; Brown, R.S.; Daniels , J.R.; and Fullmer, H.M.; Human Granulocyte Collagenase, Science159:1483-1485, 1968.
12.
Attstrom, R. : Studies on Neutrophil Polymorphonuclear Leukocytes at the Dentogingival Junction in Gingival Health and Disease, J Periodont Res (suppl)8:7-15, 1971.
13.
Robertson, P.B.; Ryel, R.B., Fullmer , H.M.; Taylor, R.E.: and SHYU, K.W.: Polymorphonuclear Leukocyte Collagenase: Localization in Granules Subjected to Zonal Centrifugation, J Oral Pathol1:103-107, 1972.
14.
Ohlsson, K.; Olsson, I.; and Tynelius-Bratthall, G.: Neutrophil Leukocyte Collogenase, Elastase and Serum Protease Inhibitors in Human Gingival Crevices, Acta Odontol Scand31:51-59, 1973.
15.
Taylor, A.C. : Collagenolysis in Cultured Tissue: II. Role of Mast Cells, J Dent Res50:1301-1306, 1971.
16.
Robertson, P.B. ; Grupe, H.E., JR. ; Taylor , R.E.; Shyu, K.W.; and Fullmer, H.M.: The Effect of Collagenase-Inhibitor Complexes on Collagenolytic Activity of Normal and Inflamed Gingival Tissue, J Oral Pathol2: 28-32, 1973.
17.
Reddick, M.E. ; Bauer, E.A.; and Eisen, A.Z.: Immunocytochemical Localization of Collagenase in Human Skin and Fibroblasts in Monolayer Culture , J Invest Dermatol62: 361-366, 1974.
18.
Hook, R.M.; Hook, C.W.; and Brown, S.L.: Fibroblast Collagenase Partial Purification and Characterization, Invest Ophthalmol12:771-776, 1973.
19.
Leighton, J. ; Mark, R.; and Justh, G.: Patterns of Three-Dimensional Growth In Vitro in Collagen-Coated Cellulose Sponge: Carcinomas and Embryonic Tissues, Cancer Res28:286-295, 1968.
20.
Rose, G.G.: A Separable and Multipurpose Tissue Culture Chamber, Tex Rep Biol Med12:1074-1083, 1954.
21.
Rose, G.G.; Pomerat, C.M. ; Shindler, T.O.; and Trunnell, J.B.: A Cellophane-Strip for Culturing Tissue in Multipurpose Culture Chambers , J Biophys Biochem Cytol4:761-764, 1958.
22.
Rose, G.G.: Cytopathophysiology of Tissue Cultures Growing Under Cellophane Membranes , Int Rev Exp Pathol5:111-178, 1966.
23.
Rose, G.G. (ed) : Atlas of Vertebrate Cells in Tissue Culture, New York: Academic Press, Inc., 1970.
24.
Dingle, J.T. : Action of Vitamin A on the Stability of Lysosomes In Vivo and In Vitro, Ciba Found Symp Lysosomes, 1963, pp 384-398.
25.
Weissmann, G., and Thomas, L.: Studies on Lysosomes: II. The Effect of Cortisone on the Release of Acid Hydrolases from a Large Granule Fraction of Rabbit Liver Induced by an Excess of Vitamin A, J Clin Invest42:661-669, 1963.
26.
Franklin, D.M. , and Wynn, C.U.: The Degradation of Acid-Soluble Collagen by Rat-Liver Preparations, Biochem J85:276- 282, 1962.
27.
Page, R.C., and Schroeder, H.E.: Biochemical Aspects of the Connective Tissue Alterations in Inflammatory Gingival and Periodontal Disease, Int Dent J23:455-469, 1973.
28.
Robertson, P.B., and Simpson, J.: Collagenase : Current Concepts and Relevance to Periodontal Disease, J Periodontol47 :29-33, 1976.
