Undecalcified human dentin was prepared for electron microscopy by conventional chemical fixation with alcoholic dehydration and by freeze-substitution with cryodehydration. Comparisons are reported concerning effects of different preparation methods. Morphology remained unaltered in the pre-dentin and mineralized matrices. The technique comparison permitted inferences regarding hydration states and relative amounts of protein in different areas of the odontoblast cell.
Get full access to this article
View all access options for this article.
References
1.
Bullivant, S.: Present Status of Freezing Techniques, in Parsons, D.F. (ed) : Some Biological Techniques in Electron Microscopy, New York: Academic Press, Inc., 1970, pp 101-146.
2.
Kay, D.H. (ed) : Techniques for Electron Microscopy, 2nd ed, Philadelphia : F.A. Davis, 1965, pp 206-207.
3.
Bennett, H.S. , and Luft, J.H.: s-Collidine as a Basis for Buffering Fixatives , J Biophys Biochem Cytol6: 113-114, 1959.
4.
Reynolds, E.S. : The Use of Lead Citrate at High pH as an Electron Opaque Stain in Electron Microscopy, J Cell Biol17: 208-212, 1963.
5.
Humason, G.L.: Animal Tissue Techniques, 2nd ed, San Francisco: W. H. Freeman & Co., 1967, p 493.
6.
Frank, R.M.: Ultrastructural Relationship Between the Odontoblast, Its Process, and the Nerve Fiber, in Symons, N.B.B. (ed) : Dentin and Pulp: Their Structure and Reactions, London: E. & S. Livingstone, Ltd., 1968, pp 115-145.
7.
Frank, R.M., and Sognnaes, R.F.: Teeth, in Greep, R.O., and Weiss, L. (eds) : Histology, 3rd ed, New York: McGraw-Hill , 1973, pp 511-538.
8.
Johansen, E. , and Parks, H.F.: Electron-Microscopic Observations on Sound Human Dentin, Arch Oral Biol7: 185-193, 1962.
9.
Nalbandian, J., and Frank, R.M.: Microscopic electronique de l'odontoblaste humain et de son prolongement au cours de la dentinogenese, C R Soc Biol, (Paris) 156: 1498-1499, 1962.
10.
Noble, H.W. ; Carmichael, A.F.; and Rankine, D.M.: Electron Microscopy of Human Developing Dentine, Arch Oral Biol7: 395-399, 1962.
11.
Nylen, M.U. , and Scott, D.B.: An Electron Microscopic Study of Odontogenesis , J Indiana State Dent Assoc39: 406-421, 1960.
12.
Roane, J.B. , Foreman, D.W., Melfi, R.C.; and Marshall , F.J.: An Ultrastructural Study of Dentinal Innervation in the Adult Human Tooth, Oral Surg25: 94-104, 1973.
13.
Pease, D.D. : The Preservation of Tissue Fine Structure During Rapid Freezing, J Ultrastruct Res21:98-124, 1967.
14.
Frank, R.M. : Attachment Sites Between the Odontoblast Process and the Intradentinal Nerve Fibers, Arch Oral Biol13: 833-834, 1968.
15.
Pease, D.C. : Eutectic Ethylene Glycol and Pure Propylene Glycol as Substituting Media for the Dehydration of Frozen Tissues, J Ultrastruct Res21:75-97, 1967.
16.
Dallam, R.D. : Determination of Protein and Lipid Lost During Osmic Acid Fixation of Tissues and Cellular Particulates, J Histochem Cytochem5: 178-181, 1957.
17.
Luft, J.H., and Wood, R.L.: The Extraction of Tissue Protein During and After Fixation with Osmium Tetroxide in Various Buffer Systems, J Cell Biol19 (suppl) : 46A, 1963.
18.
Hayat, M.A.: Principles and Techniques of Electron Microscopy, Vol 1, New York: Van Nostrand Reinhold Co., 1970, pp 35-105.
19.
Sabatini, D.D. ; Bensch, K.; and Barrnett, R.J.: Cytochemistry and Electron Microscopy : The Preservation of Cellular Structure and Enzymatic Activity by Aldehyde Fixation, J Cell Biol17: 19-58, 1963.
20.
Meryman, H.T. (ed) : Cryobiology , New York: Academic Press, Inc. , 1966, p 1.
21.
Ling, G.N.: All or-None Adsorption by Living Cells and Model Protein-Water Systems: Discussion of the Problem of "Permease-Induction" and Determination of Secondary and Tertiary Structures of Proteins, Fed Proc25: 958-970, 1966.
22.
Weiss, L.: The Cell, in Greep, R.O., and Weiss, L. (ed) : Histology, 3rd ed, New York: McGraw-Hill , 1973, pp 1-83.
23.
Reith, R.J. : Collagen Formation in Developing Molar Teeth of Rats, J Ultrastruct Res21: 383-414, 1968.
24.
Sicher, H., and Bhaskar, S.N. (eds) : Orban's Oral Histology and Embryology , 7th ed, St. Louis: C. V. Mosby, 1972, pp 99-100.
