When two different strains of Streptococcus mutans, PK-1 and JC-2, were used to prepare cell lysates, a satellite band of plasmid deoxyribonucleic acid (DNA) was seen. The mutants of PK-1 and JC-2 were defective in their ability to synthesize insoluble extracellular polysaccharides and had no detectable satellite band of DNA. These mutants were induced by treatment with ethidium bromide, acridine orange, or sodium dodecyl sulfate.
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