A method is described for identifying and characterizing microorganisms of the family Bacteroidaceae by their protein profiles. Protein profiles of six species and several strains of this family were compared using gradient polyacrylamide gel electrophoresis in the slab form. The protein profiles were sufficiently distinct and reproducible to allow identification of species, subspecies, and even minor strain differences. This technique is suggested as an adjunct to the present methods of characterization and identification of these microorganisms.
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References
1.
Smith, L., and Holdeman, L.V.: The Pathogenic Anaerobic Bacteria, Springfield, Ill: Charles C Thomas, 1968.
2.
Sutter, V.L.; Attebery, H.R.; Rosenblatt, J.E.; Bricknfll, K.S.,; and Finegold, S.M.: Anaerobic Bacteriology Manual, Los Angeles: UCLA Extension Division, 1972.
3.
Theodore, R.S. ; Tully, J.G.; and Cole , R.M.: Polyacrylamide Gel Identification of Bacterial L-Forms and Mycoplasma Species of Human Origin , Appl Microbiol21 :272-277, 1972.
4.
Dyer, J.K., and Anderson, A.W.: Disc Gel Electrophoresis: An Aid in the Identification of the Various Types of Clostridium botulinum, J Dent Res50: 431, 1971.
5.
Affronti, L.R. ; Wright, G.L., JR. ; and Reich , M.: Characterization and Comparison of Mycobacterial Antigens by Discontinuous Pore Gradient Gel Electrophoresis , Infect Immun5: 474-481, 1972.
6.
Simonson L.G. , and Shklair, I.L.: Gel Electrophoresis of Intra-Cellular Components of Some Cariogenic Streptococci, J Dent Res51: 488-491, 1972.
7.
El-Sharkay, T.A., and Huisingh , D.: Differentiation Among Xanthomonas Species by Polyacrylamide Gel Electrophoresis of Soluble Proteins , J Gen Microbiol68: 155-165, 1972.
8.
Holdeman, L.V., and Moore, W.E.C.: Anaerobic Laboratory Manual, Blacksburg, Va: Virginia Polytechnic Institute and State University, 1972 .
9.
Wright, G.L. , JR.; Farrell, K.B.; and Rob-Erts , D.B.: Gradient Polyacrylamide Gel Electrophoresis of Human Serum Proteins: Improved Discontinuous Gel Electrophoretic Technique and Identification of Serum Components, Clin Chim Acta32: 285-296, 1971.
10.
Prevot, A.R.: Manual for the Classification and Determination of Anaerobic Bacteria, 1st ed (American) FREDETTE, V. (trans) , Philadelphia : Lea & Febiger, 1966.
11.
Finegold, S.M. ; Harada, N.E.; and Miller, L.G.: Antibiotic Susceptibility Patterns as Aids in Classification and Characterization of Gram-Negative Anaerobic Bacilli , J Bacteriol94: 1443-1450, 1967 .
12.
Ornstein, L. : Disc Electrophoresis: I. Background and Theory , Ann NY Acad Sci121: 321-349, 1967.
