Acrylamide gel electrophoretic methods were used to assess the numbers and types of sucrose-metabolizing enzymes elaborated by Streptococcus mutans strains HS-6, SL-1, FA-1, and NCTC 10449. Two types of polysaccharide-synthesizing enzymes and one invertaselike enzyme were distinguished. The patterns of extracellular proteins and enzymes were distinctive for each strain.
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References
1.
Gibbons, R.J. , and Nygaard, M.: Synthesis of Insoluble Dextran and Its Significance in the Formation of Gelatinuous Deposits by Plaque-Forming Streptococci, Arch Oral Biol13: 1249-1262, 1968.
2.
Guggenheim, B.: Enzymatic Hydrolysis and Structure of Water-Insoluble Glucan Produced by Glucosyltransferases from a Strain of Streptococcus mutans , Helv Odontol Acta14 (suppl V) : 89-108, 1970.
3.
Gibbons, R.J. : Presence of an Invertase-Like Enzyme and a Sucrose Permeation System in Strains of Streptococcus mutans, Caries Res6: 122-131, 1972.
4.
McCabe, M.M. , and Smith, E.E.: Invertase Activity in Streptococcus mutans and Streptococcus sanguis, Arch Oral Biol18: 525-531, 1973.
5.
Tanzer, J.M. ; Brown, A.T.; and Mcinerney , M.F.: Identification, Preliminary Characterization, and Evidence for Regulation of Invertase in Streptococcus mutans, J Bacteriol116: 192-202, 1973.
6.
Guggenheim, B., and Newbrun, E.: Extracellular Glucosyltransferase Activity of an HS Strain of Streptococcus mutans, Helv Odontol Acta13: 84-97, 1969.
7.
Carlsson, J. : A Levansucrase from Streptococcus mutans, Caries Res4: 97-113, 1970.
8.
Bratthall, D. : Demonstration of Five Serological Groups of Streptococcal Strains Resembling Streptococcus mutans, Odontol Rev21: 143-152, 1970.
9.
Bratthall, D. : Serological Studies on Streptococcus mutans , Odontol Rev23 (suppl) : 3-20, 1972.
Lowry, O.H. ; Roseborough, N.T.; Farr , A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193: 265-275, 1951.
12.
Meyer, T.S., and Lamberts, B.L.: Zone Electrophoresis of Human Parotid Saliva in Acrylamide Gel, Nature (Lond) 205 : 1215-1216, 1965.
13.
Gabriel, O., and Wanc, S.F.: Determination of Enzymatic Activity in Polyacrylamide Gels, Anal Biochem27: 545-554, 1969.
14.
Somogyi, M.: A New Reagent for Determination of Sugars, J Biol Chem160:61-68, 1945.
15.
Chrambach, A. ; Reisfeld, R.A.; Wickoff , M.; Zaccari, J.: A Procedure for Rapid and Sensitive Staining of Protein Fractionated by Polyacrylamide Gel Electrophoresis, Anal Biochem20: 150-154, 1967.
16.
Lund, B.M.: A Comparison by Use of Gel Electrophoresis of Soluble Protein Components and Esterase Enzymes of Some Group D Streptococci, J Gen Microbiol40: 413-419, 1965.
17.
Theodore, T.S. ; King, J.R.; and Cole, R.M.: Identification of L-Forms by Polyacrylamide Gel Electrophoresis, J Bacteriol97: 495-499, 1969.
Brown, A.T. , and Patterson, C.E.: Heterogeneity of Streptococcus mutans Strains Based on their Mannitol-1-Phosphate Dehydrogenases. Criterion for Rapid Classification , Infect Immun6: 422-424, 1972.
20.
Simonson, L.G. , and Shklair, I.L.: Gel Electrophoresis of Intracellular Components of Some Cariogenic Streptococci, J Dent Res51 :488-491, 1972.
21.
Berman, K.S. , and Gibbons, R.J.: Iodophilic Polysaccharide Synthesis by Human and Rodent Oral Bacteria, Arch Oral Biol11 :533-542, 1969.
22.
Carlsson, J. : Nutritional Requirements of Streptococcus mutans , Caries Res4: 305-320, 1970.
23.
Lawson, J.W. : Growth of Cariogenic Streptococci in Chemically Defined Medium, Arch Oral Biol16:339-343, 1971.
24.
Carlsson, J. , and Elander, B.: Regulation of Dextransucrase Formation by Streptococcus sanguis, Caries Res7: 89-101, 1973.