An in vitro method that uses thawing frozen saliva offers an improved way for studying the interactions of food and other factors in the initiation of caries.
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References
1.
I. Pigman, W.; Elliot, H.C.; and Laffre, R.O.: An Artificial Mouth for Caries Research, J Dent Res31:627-633, 1952.
2.
Wagg, B.J. ; Melville, T.H.; and Hartles, R.L.: A Technique for Studying the Microorganisms with Extracted Teeth Under Continuous Irrigation, Br Dent J103: 121-124, 1957.
3.
Rowles, S.L. ; Sidaway, D.A.; Macgregor , A.B.; and Marsland , E.A.: An Apparatus for the Production of Caries In Vitro, Arch Oral Biol8: 311-318, 1963.
4.
Jordan, H.V. , and Keyes, P.H.: In Vitro Methods for the Study of Plaque Formation and Carious Lesions, Arch Oral Biol11:793-801, 1966.
Yaari, A.: Experimental Caries System Using Thawed Saliva, MS thesis, University of Rochester, 1973.
7.
Yurchenco, J.A. ; Piepoli, C.R.; and Yur-Cheno , M.O.: Low Temperature Storage for Maintaining Stable Infectious Bacterial Pools, Appl Microbiol2: 53-55, 1954.
8.
Mazur, P., and Schmidt, J.J.: Interactions of Cooling Velocity, Temperature and Warming Velocity on the Survival of Frozen and Thawed Yeast, Cryobiology5: 1-17, 1968.
9.
Mazur, P.: Physiological and Chemical Basis of Injury in Single-Celled Microorganisms Subjected to Freezing and Thawing, in Meryman, H.T. (ed) : Cryobiology, New York: Academic Press, Inc., 1966, p 213.
10.
Bibby, B.G. : Determination of Percentage Occurrence of Various Groups of Mouth Bacteria, J Dent Res17: 265-272, 1938.
11.
Theilade, E. ; Wright, W.H.; Jensen , S.B., and Loe , H.: Experimental Gingivitis in Man: II. A Longitudinal Clinical and Bacteriological Investigation, J Periodont Res1:1-13, 1966.
12.
Howell, A.; Rizzo, A.; and Poul, F.: Cultivable Bacteria in Developing and Mature Human Dental Calculus , Arch Oral Biol10: 307-313, 1965.
13.
Ritz, H.L.: Microbial Population Shifts in Developing Human Dental Plaque, Arch Oral Biol12: 1561-1568, 1967 .
14.
Darling, A.I. : Studies of the Early Lesion of Enamel Caries , Br Dent J105: 119-135, 1958.
