A perifusion chamber technique is described that permits continuous measurement of the in vitro release of salivary enzymes from slices of mouse salivary glands responding to various stimuli. Kinetics of amylase secretion differed from those of esterase, which suggests that the secretory processes differ for these two enzymes.
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References
1.
Smith, J.J.; Frammer, J.; and Schiff, R.: Localization and Onset of Amylase Activity in Mouse Salivary Glands as Determined by a Substrate Film Method, J Histochem Cytochem19: 310-319, 1971.
2.
Ekfors, T.O. ; Malmihorju, T.; and Hopsu-Havu , V.K.: Isolation of Six Trypsin-Like Esteropeptidases from the Mouse Submandibular Gand, Enzymologia43: 151-165, 1972.
3.
Cutler, L.S. , and Chaudhry, A.P.: Release and Restoration of the Secretory Granules in the Convoluted Granular Tubules of the Rat Submandibular Gland , Anat Rec176: 405, 1973.
4.
Umbreit, W.W.; Burris, R.H.; and Stauffer, J.F.: Manometric and Biochemical Techniques;Minneapolis: Burgess Publishing Co., 1972, p 146.
5.
Bernfeld, P.: Amylases, a and β in Colowick, S.P., and Kaplan, N.O. (eds) : Methods in Enzymology, New York: Academic Press, Inc, 1955, p 149.
6.
Ueha, T.; Catanzaro, O.; Hanson, R.; and Lindsay, R.: Metabolic Alterations Accompanying a-Amylase Secretion by Rat Parotid Tissue In Vitro, Am J Physiol220: 312-318, 1971.
7.
Lindsay, R.H. ; Ueha, T.; Hulsey, B.; and Hanson, R.: Relationship of Chemically Initiated Secretion of Metabolism in Rat Parotid In Vitro, Am J Physiol221: 80-85, 1971.
8.
Carlsoo, B.; Danielsson, A.; Marklund, S.; and Stigbrand, T.: Simultaneous Release of Amylase and Peroxidase from the Guinea Pig Submandibular Gland, FEBS Lett25: 69-72, 1972.
9.
Bdolah, A.: The Mechanism of Enzyme Secretion by the Cell: II. Secretion of Amylase and Other Proteins by Slices of Rat Parotid Gland, Arch Biochem Biophys104: 58-65, 1968.
10.
Babad, H.; Ben-Zvi, R.; Bdolah, A.; and Schramm, M.: The Mechanism of Enzyme Secretion by the Cell: IV. Effects of Inducers, Substrates, and Inhibitors on Amylase Secretion by Rat Parotid Slices, Eur J Biochem1:96-101, 1967.
11.
Shear, M.: Substrate Film Techniques for the Histochemical Demonstration of Amylase and Protease in Salivary Glands, J Dent Res51: 368-380, 1972.
12.
Hendry, I.A. : Developmental Changes in Tissue and Plasma Concentrations of the Biologically Active Species of Nerve Growth Factor in the Mouse by Using a Two-Site Radioimmuno-Assay, Biochem J128: 1265-1272, 1972.
13.
Matthews, R.W. : Studies of the Granular Convoluted Tubules in the Rat Submandibular Gland, J Dent Res53 (Special Issue) : Abstract No. 356, 1974.
14.
Riekkinen, P.J.; Ekfors, T.O.; and Hopsu-Havu , V.K.: Purification and Characterization of an Alkaline Protease from Rat Submandibular Gland, Biochim Biophys Acta118: 604-620, 1966.
15.
Oya, H. ; Nagatsu, I.; and Nagatsu, T.: Purification and Properties of Glycylprotyl-β-Naphthylamidase in Human Submaxillary Gland, Biochim Biophys Acta258: 591-599, 1972.
16.
Schneyer, C.A. : Modification of the Action of Pilocarpine by Adrenergic Blocking Agents, Proc Soc Exp Biol Med120: 230-232, 1965.
17.
Schneyer, C.A. , and Hall, H.D.: Autonomic Pathways Involved in a Sympathetic-Like Action of Pilocarpine on Salivary Composition, Proc Soc Exp Biol Med121: 96-101, 1966.
18.
Fritz, M.E. : Cationic Dependence of Resting Membrane Potentials of Parotid Acinar Cells, Am J Physiol223: 644-647, 1972.
19.
Bdolah, A., and Schramm, M.: Factors Controlling the Rate of Enzyme Secretion by the Rat Parotid Slice , Biochem Biophys Res Commun8: 266-270, 1962.
