Soluble proteins derived from a centrifuged and filtered granule-rich fraction of homogenized rat submandibular gland were analyzed by gel filtration, ion-exchange chromatography, and polyacrylamide gel electrophoresis. Both the granule-rich fraction and final supernatant fraction contained alkaline esterase activity. The major protein component, derived from granules of the convoluted tubules, was further resolved into a series of peptides ranging in molecular weight from 9,000 to 55,000 daltons.
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References
1.
Shackleford, J.M., and Klapper, C.E.: Casein Degrading Ability of Hamster, Rat and Mouse Salivary Glands, Arch Oral Biol7: 337, 1962.
2.
Attardi, D.G. ; Levi-Montalcini, R.; Wenger, B.S.; and Angeletti , P.U.: Submaxillary Gland of Mouse: Effect of Fraction on Tissues of Mesodermal Origin In Vitro, Science150: 1307, 1965.
3.
Angeletti, R.A. ; Angeletti, P.U.; and Calissano , P.: Testosterone Induction of Esteroproteolytic Activity in the Mouse Submaxillary Gland, Biochim Biophys Acta139: 372, 1972.
4.
Attardi, D.G. ; Schlesinger, M.Z.; and Schlesinger , S.: Submaxillary Gland of Mouse:Properties of a Purified Protein Affecting Muscle Tissue In Vitro , Science156: 1253, 1967.
5.
Ekfors, T.O. ; Riekkinen, P.J.; Malmiharju, T.; and Hopsu-HAvu , V.K.: Four Isozymic Forms of a Peptidase Resembling Kallekrein Purified from the Rat Submandibular Gland , Hoppe-Seyler's Z Physiol Chem348: 111, 1967 .
6.
Hopsuhavu, V.K. ; Riekkinen, P.J.; and Ekfors, T.O.: Studies on the Alkaline Trypsin-Like Enzymes in Rat Submandibular Gland and Saliva, Acta Odontol Scand25: 657, 1967.
7.
Riekkinen, P.J. ; Ekfors, T.O.; and Hopsuhavu , V.K.: Purification and Characteristics of an Alkaline Protease from Rat Submandibular Gland, Biochim Biophys Acta118: 604, 1966.
8.
Minato, A.; Hirose, S.; and Hayashi, S.: Studies on Proteases in Submaxilliary Gland: I. Fractionation and General Characteristics of Rat Submaxillary Proteases, Chem Pharm Bull (Tokyo) 15: 1356, 1967.
9.
Riekkinen, P.J. ; Ekfors, T.O.; Hollmen, T.; and Hopsuhavu, V.K.: Purification and Physical Characteristics of a Trypsin-Like Protease from the Rat Submandibular Gland, Enzymology32: 97, 1967.
10.
Riekkinen, P.J.; Ekfors, T.O.; and Hopsuhavu , V.K.: Substrate Specificity and Modifier Characteristics of Alkaline Protease from the Rat Submandibular Gland :Glandulain, Enzymology32: 110, 1967.
11.
Calissano, P., and Anceletti, P.U.: Testosterone Effect on the Synthetic Rate of Two Esteropeptidases in the Mouse Submaxillary Gland, Biochim Biophys Acta156: 51, 1968.
12.
Schenkein, I.; Boesman, M.; Tokarsky, E.; Fishman, L.; and Levy, M.: Proteases from Mouse Submaxillary Gland, Biochem Biophys Res Commun36: 156, 1969.
13.
Ekfors, T.O.; Malmiharju, T.; and Hopsuhavu, V.K.: Isolation of Six Trypsin-Like Esteropeptidases from the Mouse Submandibular Gland, Enzymology43: 151, 1972.
14.
Pasquini, F.; Petris, A.; Sbaraglia, G.; Scopelliti, R.; Cenci, G.; and Frati, L.: Biological Activities in the Granules Isolated from the Mouse Submaxillary Gland, Exp Cell Res86: 233, 1974.
15.
Chiang, T.S.; Erdös, E.G.; Miwa, I.; Tague, L.L.; and Coalson, J.J.: Isolation from a Salivary Gland of Granules Containing Renin and Kallekrein , Circ Res23: 507, 1968.
16.
Erdös, E.G.; Tague, L.L.; and Miwa, I.: Kallekrein in Granules of the Submaxillary Gland, Biochem Pharmacol17: 667, 1968.
17.
Hackenthal, E. ; Koch, C.; Bergemann, T.; and Gross, F.: Partial Purification and Characterization of a Renin-Like Enzyme from Rat Submandibular Gland, Biochem Pharmacol21: 2779, 1972.
18.
Hanks, C.T. , and Chakrabarti, S.G.: Biochemical and Morphological Studies of Rat Submandibular GlandI. Centrifugal Fractionation of Granule-Rich Fraction, J Dent Res54: 938-947, 1975.
19.
Lowry, O.H. ; Rosenbrough, N.J.; Fair, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193: 265, 1951.
20.
Kapitany, R.A. , and Zebrowski, E.J.: High Resolution PAS Stain for Polyacrylamide Gel Electrophoresis, Anal Biochem56: 361, 1973.
21.
Campbell, D.H.; Garvey, J.S.; Cremer, N.E.; and Sussdorf, D.H.: Methods in Immunology, New York: W. A. Benjamin Publishers, 1964, pp 118-122.
22.
Peterson, E.A. , and Sober, H.A.: Chromatography of Proteins: I. Cellulose Ion-Exchange Adsorbents, J Am Chem Soc78: 751, 1956.
23.
Nakane, P.K. , and Pierce, G.B.: Enzyme-Labeled Antibodies: Preparation and Application for the Localization of Antigens, J Histochem Cytochem14: 929, 1967.
24.
Stefanini, M.; Demartino, C.; and Zamboni, L.: Fixation of Ejaculated Spermatozoa for Electron Microscopy, Nature (Lond) 216: 173, 1967.
25.
Nakane, P.K.: Personal communication.
26.
Ekfors, T.O. , and Hopsu-HAvu, V.K.: Properties of the Esteropeptidases Purified from the Mouse Submandibular Gland, Enzymology43: 177, 1972.
27.
Ekfors, T.O. , and Hopsu-Havu, V.K.: Immunofluorescent Localization of Trypsin-Like Esteropeptidases in the Mouse Submandibular Gland, Histochem J3: 415, 1971.
28.
Turkington, R.W.; Males, J.L.; and Cohen, S.: Synthesis and Storage of Epithelial-Epidermal Growth Factor in Submaxillary Gland, Cancer Res31: 252, 1971.
29.
Cutler, L.S. , and Chaudhry, A.P.: Release and Restoration of the Secretory Granules in the Convoluted Granular Tubules of the Rat Submandibular Gland , Anat Rec176: 405, 1973.
30.
Palade, G.E. : Intracisternal Granules in the Exocrine Cells of the Pancreas, J Biophys Biochem Cytol2: 417, 1956.
31.
Warshawsky, H.; Leblond, C.P.; and Droz, B.: Synthesis and Migration of Proteins in the Cells of the Exocrine Pancreas as Revealed by Specific Activity Determination from Radioautographs, J Cell Biol16: 1, 1963.
32.
Levi-Montalcini, R.: Effects of Mouse Tumor Transplantation on the Nervous System, Ann NY Acad Sci555: 330, 1952 .
Green, L.A. ; Shooter, E.M.; and Varon, S.: Enzymatic Activities of Mouse Nerve Growth Factor and Its Subunits, Proc Natl Acad Sci, USA60: 1383, 1968.
35.
Naughton, M.A. ; Koch, J.; Hoffman, H.; Bender, V.; Hagopian, H.; and Hamilton, E.: Isolation and Activity of a Thymocyte-Transforming Factor from the Mouse Submaxillary Gland, Exp Cell Res57: 95, 1969.
36.
Grossman, A. ; Lele, K.P.; and Sheldon, J.: The Effect of Esteroproteases from Mouse Submaxillary Gland on the Growth of Rat Hepatoma Cells in Tissue Culture, Exp Cell Res54: 260, 1969.
37.
Jones, R.O. , and Ashwood-Smith , M.R.: Some Preliminary Observations on the Biochemical and Biological Properties of an Epithelial Growth Factor, Exp Cell Res59: 161, 1970.
38.
Varon, S.; Namura, J.; and Shooter, E.M.: Reversible Dissociation of the Mouse Nerve Growth Factor Protein into Different Subunits, Biochem7: 1296, 1968.
39.
Greene, L.A. ; Tomita, J.T.; and Varon, S.: Growth Stimulating Activities of Mouse Submaxillary Esteropeptidases on Chick Embryo Fibroblasts in Vitro, Exp Cell Res64: 387, 1971.
40.
Frazier, W.A. ; Angeletti, R.H.; and Brad-Shaw , R.A.: Nerve Growth Factor and Insulin, Science176: 482, 1972.
41.
Young, M.; Oger, J.; Blanchard, M.H.; Asdourian, H.; Amos, H.; and Arnason, B.G.: Secretion of a Nerve Growth Factor by Primary Chick Fibroblast Cultures, Science187: 361, 1975.
42.
Burnham, P. ; Raiborn, C.; and Varon, S.: Replacement of Nerve-Growth Factor by Ganglionic Non-Neuronal Cells for the Survival in Vitro of Dissociated Ganglionic Neurons , Proc Natl Acad Sci, USA69: 3556, 1972.
