The dynamics of glycoprotein biosynthesis in rabbit maxillary and palatal explants at the time of palatal fusion was studied using3H-fucose and 14C-leucine as precursors. The results show that there is an increase in glycoprotein biosynthesis in the rabbit palate in vitro at a time commensurate with the onset of contact and adherence of the palatal shelves.
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References
1.
Pourtois, M. : La Fusion des Cretes Palatines et son Alteration par Quelques Agents Teratogenes, Arch Oral Biol79: 1-75, 1968.
2.
Pourtois, M.: The Fate of Rat Palatal Shelves Cultivated in the Presence of Periodic Acid , Arch Oral Biol16: 503-508, 1970.
3.
Mato, M.; Smiley, G.R.; and Dixon, A.D.: Epithelial Changes Prior to Contact of Human Palatal Processes, IADR Program and Abstracts of Papers, No. 605, 1972.
4.
Depaola, D.P.; Miller, S.A.; Drummond, J.F.; Lorente, C.A.; and Alverez, C.J.: The Rabbit: An In Vitro Model to Study the Molecular Events Associated with Palate Fusion, J Dent Res53: 500, 1974.
5.
Pratt, R.M.; Gibson, W.; and Hassell, J.: Conconavalin A Binding to the Secondary Palate of the Embryonic Rat, J Dent Res52 (Special Issue) : Abstract No. 210, 1973.
6.
Pratt, R.M.; Goggins, J.F.; Wilk, A.L.; and King, C.T.: Acid Mucopolysaccharide Synthesis in the Secondary Palate of the Developing Rat at the Time of Rotation and Fusion, Dev Biol32: 230-237, 1973.
7.
Depaola, D.P. ; Drummond, J.F.; and Miller, S.A.: Radioautographic Study Demonstrating 3H-Fucose Uptake into Cultured Rabbit Palates at the Time of Palate Fusion, J Dent Res54: 912, 1975.
8.
Roseman, S.: Chemistry and Metabolism of Sphingolipids, in Sweely, C.C. (ed) : Chemistry and Physics of Lipids, Amsterdam: North-Holland, 1970.
Spiro, R.G.: Analysis of Sugars Found in Glycoproteins, in Neufeld, E., and Ginsberg, V. (eds) : Methods in Enzymology, New York: Academic Press, 1966.
11.
Margolis, R.K. , and Gomez, Z.: Rapid Turnover of Fucose in the Water Soluble Glycoproteins of Brain, Biochim Biophys Acta313:226-228, 1973.
12.
Lowry, 0.; Rosebrough, N.; Farr, A.L.; and Randall, R.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193: 265-275, 1951.
13.
Dische, Z., and Shettles, L.B.: A Specific Color Reaction of Methylpentoses and a Spectrophotometric Micromethod for their Determination, J Biol Chem175: 595-603, 1948.
14.
Margolis, R.K. , and Marcolis, R.U.: Disposition of Fucose in Brain, J Neurochem19: 1023-1030, 1972.
15.
Bocci, V., and Winzler, R.J.: Metabolism of L-Fucose-1-14C and of Fucose Glycoproteins in the Rat, Am J Physiol216: 1337-1342, 1969.
16.
Gehrke, C.W. , and Stalling, D.L.: Quantitative Analysis of the Twenty Natural Protein Amino Acids by Gas-Liquid Chromatography, Separation Sci2: 101-138, 1967.
17.
Heath, E.C. : Complex Polysaccharides, Ann Rev Biochem40: 41-53, 1971.
18.
Kaufman, R.L. , and Ginsburg, V.: The Metabolism of L-Fucose by HeLa Cells , Exp Cell Res50: 127-132, 1968.
19.
Coffey, J.W. ; Miller, O.N.; and Sellinger , O.Z.: The Metabolism of L-Fucose in the Rat, J Biol Chem239: 4011-4017, 1964.
20.
Ginsburg, V. , and Neufeld, E.F.: Complex Heterosaccharides of Animals, Ann Rev Biochem38: 371-388, 1969.
21.
Depaola, D.P. ; Miller, S.A.; and Drummond , J.F.: The Biochemical Growth and Development of the Palate, Maxilla, Mandible and Tongue in the New Zealand White Rabbit, Arch Oral Biol18: 1113-1119, 1973.
22.
Vargas, V.I. ; Najileti, C.; and Ascurra, J.: Cytodifferentiation of the Mouse Secondary Palate In Vitro: Morphological, Biochemical and Histochemical Aspects, J Embryol Exp Morphol27:413-430, 1972.
23.
Smiley, G.R. , and Koch, W.E.: The Fine Structure of Mouse Secondary Palate Development In Vitro, J Dent Res50: 1671-1677, 1971.
24.
Kent, P.W.: Acylation of Amino-Sugar Intermediates and the Regulation of Epithelial Glycoprotein Biosynthesis, Expos Annu Biochim Med30:97-120, 1970.
25.
Hayward, A.F. : Ultrastructural Changes in the Epithelium During Fusion of the Palatal Processes in Rats, Arch Oral Biol14 : 661-678, 1969.
26.
Deangelis, V., and Nalbandian , J.: Ultra-structure of Mouse and Rat Palatal Processes Prior to and During Secondary Palate Formation , Arch Oral Biol13: 601-608, 1968.
27.
Cook, G.M.W. : Glycoproteins in Membranes, Biol Rev43: 363-391, 1968.
28.
Glossman, H. , and Neville, D.M.: Glycoproteins of Cell Surfaces, J Biol Chem246: 6339-6346, 1971.
29.
Hart, J.C.; Smiley, G.R.; and Dixon, A.D.: Sagittal Growth Trends of the Craniofacial Complex During Formation of the Secondary Palate in Mice, Teratology6: 43-50, 1972.