Two layers of carious dentin from extracted human teeth were observed with an electron microscope. The first layer, which is superficial and fuchsin-stainable, showed degenerated collagen fibers and granular or leaflike inorganic crystals irregularly scattered. The second layer, which is profound and fuchsinunstainable, showed expanded odontoblastic processes, sound collagen fibers, and apatite crystcls bound to the fibers like fringes.
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3.
Fusayama, T. ; Okuse, K.; and Hosoda, H.: Relationship Between Hardness, Discoloration and Microbial Invasion in Carious Dentin, J Dent Res45: 1033, 1966.
4.
Terashima, S. ; Watanabe, M.; Kurosaki, N.; and Kono, A.: Hardness of Dentin Remaining after Clinical Excavation of Soft Dentin, Jap J Conservative Dent11: 115, 1969.
5.
Fusayama, T. , and Kurosaki, N.: Structure and Removal of Carious Dentin, Int Dent J22:401, 1972.
6.
Kato, S., and Fusayama, T.: Recalcification of Artificially Decalcified Dentin In Vivo, J Dent Res49: 1061, 1970.
7.
Kurosaki, N. , and Fusayama, T.: Penetration of Elements from Amalgam into Dentin, J Dent Res52: 309, 1973.
8.
Fusayama, T. , and Terashima, S.: Differentiation of Two Layers of Carious Dentin by Staining, J Dent Res51: 866, 1972.
9.
Ohgushi, K.: Collagen Fibers in the Two Layers of Carious Dentin: Histochemical Study, J Jap Stomatol Soc40: 65, 1973.
10.
PLANK and RYCHLo, cited by Sano, U.: Technic for Histology, 3rd ed, Tokyo: Nanzando Co., 1970, p 319.
11.
Nishsda, M. : Effect of Indirect Pulp Capping Material Containing SrF2 on Remineralization of Artificially Decalcified Dentin, Shonishikagaku Zasshi9: 47, 1971.
12.
Eda, S. , and Takuma, S.: Microstructure of the Peritubular Matrix in Horse Dentin, Bull Tokyo Dent College, 6: 1, 1965.
13.
Takuma, S., and Kurahashi, Y.: Electron Microscopy of Various Zones in a Carious Lesion in Human Dentine , Arch Oral Biol, 17: 439, 1962.
14.
Johansen, E. , and Parks, H.F.: Electron-Microscopic Observations on Soft Carious Human Dentin, J Den Res40: 235, 1961.
15.
Frank, R.M. : The Ultrastructure of the Tooth from the Point of View of Mineralization, Demineralization and Remineralization, Int Dent J17: 661, 1968.
Herting, H.C. : Elektronenmikroskopische Beobachtungen an kariosem Dentin: II. Mitteilung, Dtsch Zahnaerztl Z21: 1085, 1966.
18.
Johnson, N.W. ; Taylor, B.R.; and Berman, D.S.: The Response of Deciduous Dentine to Caries Studied by Correlated Light and Electron Microscopy, Caries Res3: 348, 1969.
19.
Symons, N.B.B. : Electron Microscopic Study of the Tubules in Human Carious Dentine, Arch Oral Biol, 15: 239, 1970.
20.
Takuma, S.; Sunohara, H.; Sekiguchi, K.; and Egawa, I.: Electron Microscopy of Carious Lesions in Human Dentin, Bull Tokyo Dent College8: 143, 1967.
21.
Beust, T.B. : Resistance to Caries, J Dent Res11:619, 1931.
22.
Bradford, E.W. : The Dentin: A Barrier to Caries, Br Dent J109: 387, 1960.
23.
Furseth, R. , and Mjor, I.A.: The Fine Structure of Corticosteroid-Covered, Human Dentine, Arch Oral Biol17: 719, 1972.
24.
Sato, Y.: Removal of Infected Dentin Using Fuchsin Staining as a Guide (with Extracted Carious Teeth) , J Jap Stomatol Soc40: 420, 1973.
25.
Sato, Y.: Removal of Infected Dentin Using Fuchsin Staining as a Guide (with Vital Carious Teeth) , J Jap Stomatol Soc41: 202, 1974.
