The solubilities of extracellular polysaccharide fractions produced by 14 oral streptococcal strains were compared in water, aqueous lithium and guanidine salt solutions, dimethyl sulfoxide (DMSO), and a 9:1 DWSO-water mixture. The best results for solubilizing the fractions were obtained with the DMSO-water mixture procedure, which used the water and DMSO in sequence. By this method all of the fractions were solubilized.
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References
1.
Guggenheim, B., and Schroeder, H.E.: Biochemical and Morphological Aspects of Extracellular Polysaccharides Produced by Cariogenic Streptococci, Helv Odontol Acta11: 131-152, 1967.
2.
Gibbons, R.J. , and Nygaard, M.: Synthesis of Insoluble Dextran and Its Significance in the Formation of Gelatinous Deposits by Plaque-Forming Streptococci, Arch Oral Biol13: 1249-1262, 1968.
3.
Lobene, R.R. : A Clinical Study of the Effect of Dextranase on Human Dental Plaque, JADA82: 132-135, 1971.
4.
Guggenheim, B.: Enzymatic Hydrolysis and Structure of Water-Insoluble Glucan Produced by Glucosyltransferases from a Strain of Streptococcus mutans , Helv Odontol Acta14 (Suppl V) : 89-108, 1970.
5.
Schroeder, H.E. , and De Boever, J.: The Structure of Microbial Dental Plaque , in McHugh, W.D. (ed) : Dental Plaque, Edinburgh : E. & S. Livingston Ltd, 1970, pp. 49-75.
6.
McDougall, W.A.: Studies on the Dental Plaque: IV. Levans and the Dental Plaque, Aust Dent J9:1-5, 1964.
7.
Critchley, P.; Wood, J.M.; Saxton, C.A.; and Leach, S.A.: The Polymerisation of Dietary Sugars by Dental Plaque, Caries Res1 : 112-129, 1967.
8.
Wood, J.M.: The Amount, Distribution and Metabolism of Soluble Polysaccharides in Human Dental Plaque, Arch Oral Biol12: 849-858, 1967.
9.
Guggenheim, B.; Regolati, B.; and Muhle-Mann, H.R. : Caries and Plaque Inhibition by Mutanase in Rats, Caries Res6: 289-297, 1972.
10.
Ceska, M.; Granath, K.; Norrman, B.; and Guggenheim, B.: Structural and Enzymatic Studies on Glucans Synthesized with Glucosyltransferases of Some Strains of Oral Streptococci, Acta Chem Scand26 : 2223-2230, 1972.
11.
Baird, J.K. ; Longyear, M.C.; and Ellwood, D.C.:- Water Insoluble and Soluble Glucans Produced by Extracellular Glycosyltransferases from Streptococcus mutans, Microbios8:143-150, 1973.
12.
Jayme, G., and Neuschaffer, K.: Cadmiumaminkomplexbasen als Losungsmittel fur Cellulose, Makromolekulare Chem23: 71-83, 1957.
13.
Moye, C.J.: Non-Aqueous Solvent for Carbohydrates, in Tipson, R.S., and Horton, D. (eds) : Advances in Carbohydrate Chemistry and Biochemistry, Vol 27, New York: Academic Press Inc., 1972 , pp 85-125.
14.
Adkins, G.K. , and Greenwood, C.T.: Studies on Starches of High Amylose Content: An Improved Method for the Fractionation of Maize and Amylomaize Starches by Complex Formation from Aqueous Dispersion after Pretreatment with Methyl Sulphoxide, Carbohydr Res11 :217-224, 1969.
15.
Whistler, R.L. , and Bemiller, J.N.: Alkaline Degradation of Polysaccharides , Advan Carbohydr Chem13: 289-329, 1958.
16.
Jeanes, A.: Northern Regional Research Laboratory, Peoria, Ill, personal communication , 1971.
17.
Dintzis, F.R. ; Tobin, R.; and Babcock, G.E.: Amylose Optical Rotation in Some Mixed Solvent Systems, Biopolymers10: 379-389, 1971.
18.
Erlander, S.R. , and Tobin, R.: The Mechanism of Dispersion of Starch by Urea and by Guanidinium and Lithium Salts, Makromolekulare Chem107: 204-221, 1967.
19.
Leach, H.W. , and Schoch, T.J.: Structure of the Starch Granule. III. Solubilities of Granular Starches in Dimethyl Sulfoxide, Cereal Chem39: 318-327, 1962.
20.
Erlander, S.R. , and Tobin, R.: The Stability of the Helix of Amylose and Amylopectin in DMSO and Water Solutions, Makromolekulare Chem111: 194-211, 1968.
21.
Dintzis, F.R. , and Tobin, R.: Optical Rotation of Some a-1, 4-Linked Glucopyranosides in the System H2O-DMSO and Solution Conformation of Amylose, Biopolymers7 : 581-593, 1969.
22.
Antonini, E. ; Bellelli, L.; Bruzzesi, M.R.; Caputo, A.; Chiancone, E.; and Rossi-Fanneli , A.: Studies on Dextran and Dextran Derivatives. I. Properties of Native Dextran in Different Solvents , Biopolymers2: 27-34, 1964.
23.
Jeanes, A.: Preparation of Dextrans from Growing Leuconostoc Cultures, in Whistler, R.L. (ed) : Methods in Carbohydrate Chemistry, Vol V, New York: Academic Press, 1965, pp 118-126.
24.
Dubois, M.: GILLES, K.A.; HAMILTON, J.K.; REBERS, P.A.; and SMITH, F.: Colorimetric Method for Determination of Sugars and Related Substances, Anal Chem28: 350-356, 1956.
25.
Newbrun, E. : Extracellular Polysaccharides Synthesized by Glucosyltransferases of Oral Streptococci, Caries Res6:132-147, 1972.
26.
Chemical Rubber Co.: Handbook of Chemistry and Physics, 53rd ed, Cleveland: Chemical Rubber Co., 1972-1973, pp E-208.
27.
Somogyi, M. : A New Reagent for the Determination of Sugars , J Biol Chem160: 61-68, 1945.
28.
Lamberts, B.L. ; Meyer, T.S.; and Rozman, W.: Infrared Spectroscopic Studies of Glucans from Cariogenic Streptococci, J Dent Res53 (Special Issue) : Abstract No. 472, 1974.
