A convenient, sensitive, and reliable assay for the conversion of radiolabeled sucrose to alcohol-insoluble dextran by the Streptococcus mutans dextransucrase has been developed.
Get full access to this article
View all access options for this article.
References
1.
Schactele, C.F. ; Loken, A.E.; and Knudson, D.J.: Preferential Utilization of the Glucosyl Moiety of Sucrose by a Cariogenic Strain of Streptococcus mutans, Infect Immun5:531-536, 1972.
2.
Robrish, S.A. ; Reid, W.; and Krickevsky , M.I.: Distribution of Enzymes Forming Polysaccharides from Sucrose and the Composition of Extracellular Polysaccharide Synthesized by Streptococcus mutans, Appl Microbiol24: 184-190, 1972.
3.
Evans, R.T., and Genco, R.J.: Inhibition of Glucosyltransferase Activity by Antisera to Known Serotypes of Streptococcaas mutans, Infect Immun7: 237-241, 1973.
4.
Bollum, F.J. : Thermal Conversion of Non-priming Deoxyribonucleic Acid to Primer, J Biol Chem234: 2733-2734, 1959.
5.
Thomas, J.A. ; Schlenoer, K.K.; and Larner, J.: A Rapid Filter Paper Assay for UDPglucose-glycogen Glucosyltransferase, Including an Improved Biosynthesis of UDP14C-glucose , Anal Biochem25: 486-499, 1968.
6.
Gilboe, D.P. ; Larson, K.L.; and Nuttall, F.Q.: Radioactive Method for the Assay of Glycogen Phosphorylases, Anal Biochem47: 20-27, 1972.
7.
Fitzgerald, R.J.; Keyes, P.H.; Stoudt , T.H.; and Spinell, D.M.: The Effects of a Dextranase Preparation on Plaque and Caries in Hamsters, A Preliminary Report, JADA76: 301-304, 1968.
8.
Schachtele, C.F.; Loken, A.E.; and Schmitt, M.K.: Use of Specifically Labeled Sucrose for Comparison of Extracellular Glucan and Fructan Metabolism by Oral Streptococci , Infect Immun5: 263-266, 1972.
9.
Chludzinski, A.M.; Germaine, G.R.; and Schactele , C.F.: Purification and Properties of Dextransucrase from Streptococcus mutans, J Bacteriol118: 1-7, 1974.
10.
Whelan, W.J. : Isomaltodextrins. Partial Acid Hydrolysis of Dextran, in Whistler, R.L., and Wolfrom, M.L. (eds) : Methods in Carbohydrate Chemistry, Vol 1, New York: Academic Press, 1962, pp 321-324.
11.
Whistler, R.L. , and Hickson, J.L.: Determination of Some Components in Corn Sirups by Quantitative Paper Chromatography, Anal Chem27: 1514-1517, 1955.
12.
Hough, L., and Jones, J.K.N.: Chromatography on Paper, in Whistler, R.L. , and Wolfrom, M.L. (eds) : Methods in Carbohydrate Chemistry, Vol 1, New York: Academic Press, 1962, pp 21-31.
13.
Trenel, G.; John, M.; and Dellweg, H.: Gel Chromatographic Separation of Oligosaccharides at Elevated Temperature , FEBS Lett2: 74-76, 1968.
14.
John, M.; Trenel, G.: and DELLWEG, H.: Quantitative Chromatography of Homologous Glucose Oligomers and Other Saccharides Using Polyacrylamide Gel, J Chromatogr42: 476-484, 1969.
15.
Dubois, M.; Gilles, K.A.; Hamilton, J.K.; Rebers, P.A.; and Smith, F.: Colorimetric Method for the Determination of Sugars and Related Substances , Anal Chem28: 350-356, 1956.
16.
Somogyi, M. : A New Reagent for the Determination of Sugars , J Biol Chem160: 61-68, 1945.
17.
Guggenheim, B., and Schroeder, H.E.: Biochemical and Morphological Aspects of Extracellular Polysaccharides Produced by Cariogenic Streptococci , Helv Odontol Acta11: 131-152, 1967.
18.
Gugcenheim, B.: Enzymatic Hydrolysis and Structure of Water-Insoluble Glucan Produced by Glucosyltransferases from a Strain of Streptococcus mutans , Helv Odontol Acta14: Supplement V, 89-108, 1970.