By use of immunofluorescent techniques we found oral microorganisms on the surface of the sulcular epithelium and in the epithelial and connective tissues of the gingiva of individuals with periodontal disease. Anaerobic oral microorganisms frequently were found in these areas.
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References
1.
Burnett, G.W. , and Scherp, H.W.: Oral Microbioligy and Infectious Disease , 3rd ed, Baltimore: Williams and Wilkins Co., 1968, pp 409-466.
2.
Tsutsui, M.; Utsumi, N.; and Tsubakimoto, K.: Cellular Components of Staphylococci and Streptococci in Inflamed Human Gingiva , J Dent Res47: 663, 1968.
3.
Rosan, B.: Application of Fluorescent Antibody Technic to Gingival Tissue, J Dent Res38: 700-701, 1959.
4.
Wittwer, J.W. ; Toto, P.D.; and Dickler, E.H.: Streptococcus mitis Antigens in Inflamed Gingiva, J Peridontol40:699-640, 1969.
5.
Courant, P.R. , and Bader, H.: Bacteroides melaninogenicus and Its Products in the Gingiva of Man, Periodontics4:131-136, 1966.
6.
Takeuchi, H. ; Komeda, S.; Tsubakimoto, K.; and Tsutsui, M.: Cellular Components of Bacteroides melaninogenicus in the Inflamed Human Gingiva, J Osaka Dent Univ4: 123-130. 1970.
7.
Takeuchi, H. , and Tani, A.: Cellular Components of Fusobacterium in -the Inflamed Human Gingiva, Jap J Oral Biol13: 393-401, 1971.
8.
Ford, H.S., and Burkes, E.J., JR.: Localization of Mycoplasma salivarium in Gingival Biopsies , abstracted, IADR Program of Abstracts and Papers, No. 41, 1972.
9.
Sainte-Marie, G.: A Paraffin Embedding Technique for Studies Employing Immunofluarescence, J Histochem Cytochem10: 250-256. 1962.
10.
Marshall, J.D. ; Eveland, W.C.; and Smith, C.W.: Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent Antibody Technic with a Modification of Its Application , Proc Soc Exp Biol Med98: 898-900, 1958.
11.
Taylor, R.D. : Modification of the Brown and Brenn Gram Stain for the Differential Staining of Gram-Positive and Gram-Negative Bacteria in Tissue Section, Am J Clin Pathol46: 472-474, 1966.
12.
Ouchterlony, O.: Handbook of Immunodiffusion and Immunoelectrophoresis, 2nd ed, Michigan: Ann Arbor Science Publishers, 1968, pp 1-59.
13.
Sharawy, A.M. ; Sabharwal, K.; Socransky, S.S.; and Lobene, R.L.: A Quantitative Study of Plaque and Calculus Formation in Normal Periodontally Involved Mouths, J Periodontol37: 495-501, 1966.
14.
Theilade, E. ; Wright, W.H.; Jensen, S.B.; and Löe, H.: Experimental Gingivitis in Man. II. A Longitudinal Clinical and Bacteriological Investigation, J Periodontol Res1:1-13, 1966.
15.
Rosan, B., and Williams, N.B.: Hyaluronidase Production by Oral Enterococci, Arch Oral Biol9: 291-298, 1964.
16.
Gibbons, R.J. , and Macdonald, J.B.: Degradation of Collagenous Substrates by Bacteroides melaninogenicus, J Bacteriol81: 614-625, 1961.
17.
Mergenhagen, S.E.; Hampp, E.G.; and Scherp, H.W.: Preparation and Biological Activities of Endotoxins from Oral Bacteria, J Infect Dis108: 304-310, 1961.
18.
Mergenhagen, S.E.: Endotoxic Properties of Oral Bacteria as Revealed by the Local Shwartzman Reaction, J Dent Res39 :267-272, 1960.
