Ultrastructure of Selected Bacteria Isolated from Dental Plaque as Revealed by Freeze-Etching

Abstract

Freeze-etching was used to study the ultrastructure of several isolates representative of morphologic types of bacteria found in dental plague. It is a useful technique for ultrastructural examination of apparently viable bacteria, and it has possible applications in the study of the structural appearance and interrelationships of oral bacteria in plaque.

Get full access to this article

View all access options for this article.

References

Moor, H. : Use of Freeze-etching in the Study of Biological Ultrastructure, Int Rev Exp Pathol 5: 179-216, 1966.

Koehler, J.K. : The Technique and Application of Freeze-Etching in Ultrastructure Research , Adv Biol Med Phys 12: 1-84, 1968.

Hall, D.M. , and Shroff, F.R. : The Study of Dental Ultrastructure by Freeze-Etching, Arch Oral Biol 13: 703-704, 1968.

Newman, H.N. : Structure of Organic Films Present on Enamel Surfaces In Vivo, J Dent Res 50: 1167-1168, 1971 .

Newman, H.N. : Freeze-Etching and Dental Research, J Periodont Res 7: 91-101, 1972.

Newman, H.N. : Bacteriological Interrelationships in Dental Plaque, J Dent Res 51: 1235, 1972.

Saxton, C.A. , and Findlay, J.S. : Freeze-Etching, a New Technique for Revealing the Polysaccharides in Dental Plaque, J Dent Res 50: 1174-1175, 1971 .

Staehelin, L.A. : The Interpretation of Freeze-Etched Artificial and Biological Membranes, J Ultrastruct Res 22: 326-347, 1968.

Gould, G.W. ; Stubbs, J.M. ; and King, W.L. : Structure and Composition of Resistant Layers in Bacterial Spore Coats , J Gen Microbiol 60: 347-355, 1970.

10.

Leadbetter, E.R. , and Holt, S.C. : The Fine Structure of Bacillus fastidiosus , J Gen Microbiol 52: 299-307, 1968.

11.

Holt, S.C. , and Leadbetter, E.R. : Comparative Ultrastructure of Selected Aerobic Spore-forming Bacteria: a Freeze-Etching Study, Bact Rev 33: 346-378, 1969.

12.

Kavanau, J.L. : Membrane Structure and Function, Fed Proc 25: 1096-1107, 1966.

13.

Frank, R.M. , and Brendel, A. : Ultrastructure of the Approximal Dental Plaque and the Underlying Normal and Carious Enamel, Arch Oral Biol 11: 883-912, 1966.

14.

Jones, S.J. : A Special Relationship Between Spherical and Filamentous Microorganisms in Mature Human Dental Plaque, Arch Oral Biol 17: 613-616, 1972.

15.

Poole, D.F.G. , and Newman, H.N. : Dental Plaque and Oral Health , Nature (Lond) 234: 329-331, 1971.

16.

Poole, D.F.G. , and Newman, H.N. : Topographical Variations in Dental Plaque , J Dent Res 50: 1175, 1971.

17.

Burnett, G.W. , and Scherp, H.W. : Oral Microbiology and Infectious Disease, 3rd ed, Baltimore: Williams and Wilkins Co. , 1968.

18.

Beaman, B.L. , and Shankel, D.M. : Ultrastructure of Nocardia Cell Growth and Development on Refined and Complex Agar Media, J Bact 99: 876-884, 1969.

19.

Jones, D. , and Johnson, R.P.C. : Ultrastructure of Frozen, Fractured and Etched Pycnidiospores of Coniothyrium minitans, Trans Br Mycol Soc 55: 83-87, 1970.

20.

Branton, D. : Fracture Faces of Frozen Membranes, Proc Natl Acad Sci USA 55: 1048-1056, 1966.

21.

Glauert, A.M. , and Hopwood, D.A. : The Fine Structure of Streptomyces violaceoruber (S coelicolor). III. Mycelium and Spores, J Biophys Biochem Cytol 10: 505-516, 1961.

22.

Jones, D. : Ultrastructure and Composition of the Cell Walls of Sclerotinia sclerotiorum , Trans Br Mycol Soc 54: 351-360, 1970.

23.

Young, T.W.K. : Ultrastructure of the Spore Wall of Linderina , Trans Br Mycol Soc 54: 15-25, 1970.

24.

Young, T.W.K. : Arrangement of Microfibrils in Walls of Aerial Hyphae of Linderina, Trans Br Mycol Soc 55: 29-35, 1970.

25.

Campbell, R. : Ultrastructure of an Albino Strain of Alternaria brassicola, Trans Br Mycol Soc 54: 309-313, 1970.

26.

Friedman, B.A. ; Dugan, P.R. ; Pfister , R.M. ; and Remsen, C.C. : Fine Structure and Composition of the Zoogleal Matrix Surrounding Zooglea ramigera, J Bact 96: 2144-2153, 1968.

27.

Saxton, C.A. , and Critchley, P. : An Electron Microscope Investigation of the Effect of Diminished Protein Synthesis on the Morphology of the Organisms in Dental Plaque In Vitro, in McHugh, W.D. (ed) , Dental Plaque, London: E. and S. Livingstone, 1970 , pp 109-128.

28.

Van Houte, J. , and Saxton, C.A. : Cell Wall Thickening and Intracellular Polysaccharide in Microorganisms of the Dental Plaque, Caries Res 5: 30-43, 1971.